New Applications for DLS & Zeta Potential

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00:00:00 Introduction
00:02:14 New Applications for Zetasizer Nano Series Instrumentation DLS & Zeta Potential
00:02:34 Characterization of Self-Assembly DNA-binding Surfactants
00:03:12 Outline
00:03:34 PART ONE
00:03:39 DLS and BROWNIAN MOTION
00:04:24 Dynamic Light Scattering (DLS)
00:05:19 STOKES-EINSTEIN EQUATION
00:05:47 MEASURING ELECTROPHORETIC MOBILITY
00:07:31 PART TWO
00:08:04 LIPOSOME STRUCTURE
00:09:12 LIPOSOME STRUCTURE
00:10:11 CATIONIC LIPOSOMES IN GENE THERAPY
00:11:27 PNA AMPHIPHILES (PNAA)
00:14:26 Untitled
00:16:01 PNAA MICELLE MORPHOLOGY BY SAXS
00:17:15 PNAA MICELLE SIZE USING ZETASIZER
00:18:40 TRITON MICELLE MORPHOLOGY BY SAXS
00:19:31 TRITON MICELLE SIZE USING ZETASIZER
00:20:16 PNAA Incorporation into Lipid Bilayer
00:22:01 Size distribution of polar PNA liposomes
00:23:19 Size distribution of polar PNA liposomes w/DNA
00:24:05 Mobility distribution of PNAA Lipsomes
00:24:58 Zeta Potential: DNA adsorption to PNA liposomes
00:27:33 VALIDATION OF MOBILITY FROM CAPILLARY ZONE ELECTROPHORESIS
00:29:11 Controlled rupture of liposomes by pH
00:31:49 CONCLUSIONS
00:32:57 Contact information
The Zetasizer Nano is used to characterize novel surfactant systems for DNA separations and gene delivery applications. The application requires a sensitive, low volume, label free method, so the Zetasizer is ideally suited for this