Multi-angle Dynamic Light Scattering (MADLS) offers improvements in particle sizing resolution, sensitivity and accuracy compared to traditional single angle Dynamic Light Scattering (DLS). By combining measurement data, sequentially collected, from three measurement angles it can provide improvements in resolution, typically from 3:1 to 2:1. It also provides improvements in accuracy by reducing signal to noise ratio, allowing lower smoothing coefficients to be used, and by reducing angular dependence of the final particle size result, as illustrated in the following Mie solution plot for a single protein macromolecule measured at 45, 90, and 173 degree scattering angles.


This white paper provides a technical discussion on how MADLS is implemented in the Malvern Panalytical Zetasizer Ultra system and the advantages of combining data from multiple measurement angles as well as the practical considerations when employing MADLS on your nanomaterial and biomolecular samples.




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