Protein applications for tetra detection

Log in to watch this webinar

Not registered yet? Create an account
00:00:00 Protein applications for tetra detection. Why upgrade from calibration against protein standards?
00:01:37 The Benefits of Tetra Detection for Protein Characterisation
00:02:42 GPC or SEC separates macromolecules in solution
00:04:43 Conventional Calibration
00:07:41 Limitations of conventional SEC
00:08:47 Less compact protein
00:10:44 Non-globular protein
00:12:12 Proteasome 20S filled with Cytochrome C
00:14:10 Fractionation
00:15:46 Hardware for Tetra Detection
00:17:05 Tetra Detection
00:18:03 UV Detection
00:18:34 RI Detection
00:19:33 Light Scattering detection
00:21:17 RALS/LALS Combination
00:25:09 Intrinsic Viscosity 4- capillary Differential Viscosity Detector
00:27:13 Typical Tetra Detector Chromatogram
00:28:44 Tetra Detection
00:29:10 Molecular weight measurements
00:29:37 Standard Protein – Bovine Serum Albumin
00:31:33 Standard Protein – Bovine Serum Albumin
00:31:42 Oligomer determination
00:32:50 Proteasome 20S filled with Cytochrome C
00:33:10 Protein Aggregation
00:33:37 Cases
00:33:56 FDA & USP
00:34:13 Aggregate quantification
00:35:46 Conformational changes
00:36:15 Conformational changes
00:38:10 Antibody analysis
00:38:33 Protein Conjugation
00:39:22 Co-polymer analysis
00:40:06 PEGylated Protein
00:41:06 Conjugation analysis
00:41:56 Conjugation analysis
00:42:10 Summary
00:43:07 Contact Information
There are a number of calibration techniques using protein standards for measuring protein molecular weight, the most common being conventional calibration. Why should you upgrade to add light scattering and viscosity detectors?