The Zetasizer Nano ZS is shown to measure the size of Aluminium Keggin ions of about 1nm diameter, and the zeta potential of Aluminium ion-BSA complexes. The zeta potential results support electrostatic interactions between the Al ions and BSA. The Zetasizer Nano has been successfully used to determine the size of Al13 and Al30-mers and characterize the size of complexes formed between these aluminum polyoxocations and bovine serum albumin. Measurement of the zeta potentials of Al-BSA mixtures can provide insight into the mechanisms of interaction involved in the formation of polyoxocation-protein complexes.The same interactions probably form the basis of adjuvent properties of alum.




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