Performance validation against glucose isomerase
Small-angle X-ray scattering from solutions of biological macromolecules, also known as BioSAXS, is a structural biology technique of rapidly increasing popularity. Other than with single crystal X-ray diffraction, SAXS allows to study proteins in their native state, dissolved in a suitable buffer, and crystallization is not required.Other than with single crystal X-ray diffraction, SAXS allows to study proteins in their native state, dissolved in a suitable buffer, and crystallization is not required. BioSAXS has proven as a valuable tool to analyze the overall size, compactness and aggregation behavior of protein molecules, and it can even be used to determine the 3D envelope structure at a resolution of 1-2 nm.
SAXS measurements were performed on a dilute solution of glucose isomerase. By using ScatterX78 experimental data could be acquired with good sensitivity and within short measurement times. The EasySAXS data analysis software was used to deduce information about the size, compactness, overall shape and aggregation behavior of the protein molecules. All results were found to be consistent and in good agreement with what has been reported in the scientific literature.