Determining absolute particle concentration using multi-angle dynamic light scattering (MADLS)

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00:00:00 Enhanced Precision Multi Angle DLS
00:01:06 Poll Question
00:01:06 Enhanced Precision Multi Angle DLS
00:03:47 Poll Answers
00:03:47 Enhanced Precision Multi Angle DLS
00:04:48 Brownian motion
00:06:47 Dynamic light scattering (DLS)
00:07:29 Brownian motion
00:07:37 Dynamic light scattering (DLS)
00:09:02 Correlogram deconvolution
00:10:02 Particle Size Distribution
00:11:05 Intensity to mass transform
00:13:04 DLS Results
00:14:23 Limitations – Experimental Noise
00:17:24 Intensity to mass transform
00:18:25 Limitations – Mie Scattering effects
00:22:51 Inherent Polydispersity
00:24:14 DLS Precision – Resolution Limits
00:26:48 Compounding the uncertainty
00:28:11 Intensity to mass transform
00:28:29 Compounding the uncertainty
00:30:33 Historical solution
00:32:33 Historical Solution – Ideal Samples
00:34:54 Historical solution – real samples
00:36:45 The challenge
00:38:15 The Solution
00:42:19 Adaptive Correlation
00:45:43 Multi Angle correlogram collection
00:47:26 enhanced Resolution Fitting
00:48:36 Enhanced precision size distribution
00:49:59 MADLS Example – real samples
00:54:04 MADLS – Methodology
00:55:56 Plus – particle concentration
00:56:38 Particle concentration
00:58:12 concentration – AAVs
00:59:22 Concentration – Nanoparticles
01:00:29 Particle concentration – limitations
01:04:50 Particle concentration
01:07:48 Particle concentration – range
01:09:40 Thank you for your attention
01:09:50 Question & Answer SessionAsk your question by typing within the Q & A chat facility Listening on-demand ?Send your questions
01:11:34 Particle concentration – limitations
01:14:50 Question & Answer SessionAsk your question by typing within the Q & A chat facility Listening on-demand ?Send your questions
Dynamic light scattering (DLS) is a common laboratory technique routinely used for sizing proteins, nanoparticles, polymers, & colloidal emulsions. By utilizing the improved accuracy and resolution of multi-angle dynamic light scattering (MADLS®), it is now possible to determine the absolute particle concentration distribution using the Zetasizer Ultra. This provides the user with a fast assessment of the concentration of each population of particles present within their dispersion.  In this webinar we will give an overview of the particle concentration analysis on the Zetasizer Ultra and provide some examples for liposomes and virus particles.