Knowledge Center

How the Creoptix WAVEsystem drove real-time kinetic and affinity analysis for faster and better characterization of candidates – from fragments to biologics. Bioassays, such as the enzyme-linked bioabsorbant assay (ELISA), are standard techniques in the laboratory that require enzyme or fluorescent labels for the detection of target analytes. While these label-based studies give important information about a sample, such as binding affinity, they can only measure the interaction of an analyte at a given concentration and time. This means that such assays are unable to gather kinetic analysis, for example the speed or length of binding. Yet kinetic analysis is fundamental to many stages of the drug discovery and development pipeline, from lead development to complex biologics investigation. Without this data, scientists are forced to make less informed decisions, causing delays to drug discovery and development. To improve decision-making in drug research and development, researchers need instruments that allow them to obtain high-quality kinetics data. The Creoptix WAVEsystem provides label-free interaction analysis allowing you to obtain kinetic and affinity data, alongside evaluation of binding specificity, in real-time. The system is ideal for analyzing samples based on two key patented developments: Grating-Coupled Interferometry (GCI) technology, and no-clog WAVEchip microfluidics systems.

Conventional label-based bioassays can be effective but they come with issues that can skew results. Let us show you how label-free quantification overcomes them. Find out more about how the WAVEsystem can help overcome the challenges you’re facing with conventional label-based bioassays. Login or register for free to download.

Conventional bioassays rely on enzyme or fluorescent labels to detect biomolecular interactions, but these traditional approaches come with challenges that can lead to skewed and inaccurate results. Advanced labelfree interaction analysis techniques remove many of these issues. Now, scientists can move beyond the label, getting closer to the sample and monitoring it in real-time to better understand analyte-ligand interactions and unlock deeper insight. Although label-based detection is needed to monitor some biomolecular interactions, this isn’t the case for many applications. Fast, sensitive, native, and kinetic analysis is now available to most analytical laboratories. This eBook reveals the challenges presented by conventional label-based bioassays and explores how label-free interaction analysis can deliver a world of insight through technology-driven solutions. Please login or register for free to download.

Conventional bioassays often depend on labeling to detect biomolecular interactions. Although these techniques have been used for many years, in multiple applications, they are not without their issues. The enzymes or fluorescent molecules often used as labels are not inert and can present many challenges, including non-specific binding issues. The latest label-free quantification techniques eliminate the issues presented by labels by removing them altogether, allowing the analyst to get closer to the analyte and monitor it in real-time. Interactions can now be observed as they occur so that the kinetics, as well as the affinity of the analyte-ligand relationship, can be revealed. Not only can the analyst get closer to the interaction, but they can also achieve faster and more automated results by using label-free techniques with fewer manual sample preparation or post-analysis manipulation steps. Although label-free interaction analysis offers many benefits, there are several different technologies to choose between, each with its advantages and disadvantages. Since molecules have a diverse range of characteristics and behaviors, such as sensitivity, size and complexity, these parameters must be considered when choosing a label-free technology. This eBook explores the three main label-free interaction analysis technologies, their pros and cons and their recommended applications, helping you choose the right technique for your analytics program. Please login or register for free to download.

While conventional bioassays can be effective, they come with many challenges that can skew results. Here, we highlight the alternatives to label-based bioassays. Whatever your bioassay goals there’s a label-free quantification technology to help you reach them. Find out about the benefits of each label-free technology and how the WAVEsystem can help overcome the challenges you’re facing with conventional label-based bioassays. Login or register for free to download the infographic.