Virus, Measurement of the Number of Particles in Virus Aggregates

by Seungji Kim, Monday, 21st November 2016

Measurement of Virus Particle Number
Typically, the titer of bacteriophage and virus particles is confirmed by plaque assays, and for animal cell viruses, it is confirmed by biological assays based on cell criteria.
In such array systems, infectious virus particles grow on the monolayer of cells, creating plaques (areas of destroyed cells), and the number of these plaques determines the pfu (plaque-forming units). Although this allows for counting of the infectious virus particles directly, non-infectious virus particles do not form plaques, and aggregates containing many virus particles produce only a single plaque.

Manufacturers often need to verify the number of virus particles in the pre-treatment and the degree of aggregation (as an initial indicator of limited shelf life if aggregation occurs during pre-treatment) regardless of infectivity.

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