Measurement of Protein Solution Viscosity by DLS Microrheology
In recent years, the measurement of the flow properties of high-concentration protein formulations has attracted significant attention. This application note presents an example of applying microrheology based on dynamic light scattering (DLS) to measure the complex viscosity of a model protein system.Particles embedded or dispersed in a medium undergo Brownian motion, displacing from their original position over time. The mean squared displacement (MSD) of these particles can be derived from the viscoelastic properties of the medium. That is, if the diameter of the dispersed particles is known, by measuring the MSD, information about the viscoelasticity of the sample can be obtained. In experiments using dynamic light scattering, the autocorrelation function (g) of the scattered light can be represented as a function of the MSD of the scatterers (∆r2) over time, where q indicates the scattering vector.
Subsequently, by conducting experiments using DLS, the MSD of probe particles embedded in the system of interest can be calculated. Following this, the generalized Stokes-Einstein relation (GSER) can be used to determine the complex modulus of the medium as a function of angular frequency. To continue reading, please fill out the required information in the form and download the materials.Download
Subsequently, by conducting experiments using DLS, the MSD of probe particles embedded in the system of interest can be calculated. Following this, the generalized Stokes-Einstein relation (GSER) can be used to determine the complex modulus of the medium as a function of angular frequency. To continue reading, please fill out the required information in the form and download the materials.DownloadThis article may have been translated automatically
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