Epigenetic regulation of genomic DNA for gene expression is important in cellular differentiation and the development of an organism. Epigenetics also contributes to human diseases. On a molecular level, epigenetics involves modification of the histone proteins and DNA that make up chromatin. Protein families have been identified which mediate these modifications, and characterization of the specific binding interactions are important to define the specificity and affinity of the interaction. This characterization is also important in developing drugs which inhibit these chromatin-binding proteins. This white paper summarizes how Isothermal Titration Calorimetry (ITC) is used for characterization of proteins involved in epigenetic regulation.