Size Exclusion Chromatography (SEC)

Size exclusion chromatography (SEC or SEC-HPLC) for measurement of protein absolute molecular weight, structure, size and conformation

Size Exclusion Chromatography (SEC or SEC-HPLC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.

Triple detection SEC

When SEC is coupled with light scattering, viscometer and concentration detectors (known as triple detection), it can measure absolute molecular weight, molecular size, and intrinsic viscosity and generate information on macromolecular structure, conformation, aggregation and branching.

Our systems

For polymer and protein characterization, in polymer, food, pharmaceutical and biopharmaceutical research, Malvern Panalytical offers a range of SEC systems or individual SEC detectors, including SEC-MALS, to suit your application needs.



The world’s most advanced multi-detector GPC/SEC system

Size Exclusion Chromatography (SEC)
Gel Permeation Chromatography
Measurement type
Molecular structure
Molecular size
Molecular weight