What is UPLC?
UPLC, UHPLC and HPLC are both liquid chromatography techniques to separate the components of a compound or mixture.
Ultra high performance liquid chromatography (UHPLC) and ultra performance liquid chromatography (UPLC) such as the Waters ACQUITY® APC™ are an advancement in traditional gel permeation chromatography (GPC) separations.
Using advanced hybrid particles, which are smaller than in analytical GPC columns, ACQUITY APC columns offer improved resolutions, faster separation, reduced solvent use, and utilize low-dispersion (band broadening) hardware. A follow-on benefit is that the tall narrow peaks achieved using the ACQUITY APC help to improve sensitivity and reduce detection limits.
UPLC vs HPLC
The table below highlights some of the differences between UPLC and HPLC as separation techniques.
|Typical Column dimensions||7.8 x 300 mm||4.6 x 150 mm|
|Particle size||3 - 10 µm||< 3 µm|
|Typical Run time per injection||45 minutes||10 minutes|
|Solvent usage per injection||45 mL||10 mL|
|Pump pressure||4 MPa||70 MPa|
(µg loading varies depending on sample and system set-up)
|300 µg||20 µg|
|Mobile phase change over||Normal||Fast|
Advanced detectors and UPLC
Adding multiple advanced detectors, including light scattering and viscometry to UPLC setups offers superior information about polymers and oligomers, including absolute molecular weight, size, and structure, when compared with conventional single-detector measurements.
Previously these two techniques have been incompatible due to band-broadening and dispersion effects that take place as narrow UPLC peaks pass through multiple detector cells. However, the latest enhancements to Malvern Panalytical's OMNISEC REVEAL detector platform now bring these two class-leading technologies together.
Combining the information-rich data from advanced multi-detection with the speed and resolution afforded by UPLC it is now possible to get the best of both worlds - faster and more detailed polymer analysis.