Datum der Aufzeichnung: 15 July 2010

Duration: 07 minutes 34 seconds

Aggregation in antibody formulations is of current concern because of the immunogenic response. This presentation gives examples of measurements of antibodies and their stability in various formulations at high concentration.
Table of contents
1. Formulation conditions antibody measurements
00:17
2. SEC – FDA Gold Standard
00:21
3. Protein Aggregation – Why The Concern?
00:21
4. The problem
00:20
5. Dynamic Light Scattering (DLS)
00:19
6. Batch vs. SEC Complementarity
00:34
7. SEC & DLS Pros & Cons
00:23
8. High Concentration – Issues To Consider
00:38
9. Effects, Symptoms, & Solutions
00:16
10. Antibody Formulations
00:23
11. IgG DLS Dilution Results*
00:31
12. DSL Size Distributions – Soup 1
00:28
13. DLS Size Distributions – Soup 2
00:23
14. DLS Size Distributions – Soup 3
00:12
15. DLS Size Distributions – Soup 4 (PBS)
00:17
16. DLS Size At C = 0
00:22
17. Oligomeric Distribution
00:24
18. Melting Point Comparison
00:50
19. Thank you!
00:15