Biologics – such as antibodies, nanobodies, and other large molecules manufactured in living systems – are highly complex. Characterizing them is an important task in drug development and quality control, but it can be a challenge. 

What’s more, biologic drugs often come in multiple variants, with nature and abundance heavily influenced by the manufacturing process.

To get the information you need on drug stability and performance, you need to assess the properties in relevant sample matrices.

Over the last years, label-free quantification is gaining widespread acceptance as an important research and development method, as it analyzes molecular interactions without using additional isotope or fluorescent labels.

Our newest GCI technology is a superior approach because it enables binding kinetics to be measured reliably using low sample volumes – even from unpurified material.

The Creoptix WAVEdelta is enhanced and enabled by our proprietary software wizards: 

• Ligand Screening Wizard
• CFCA Wizard

Developed with the user in mind, they drive automation, flexibility and ease of use, whether you’re working with small molecules or antibodies.  Explore the key benefits below to discover more.

Ligand Screening Wizard

A faster, flexible way to screen and characterize antibodies.

Fast-track your assay set-up

With the Ligand Screening Wizard, you can set up and run assays easily and intuitively thanks to the “ligand block”, where ligand samples are cycled through the biosensor surface in sequential capture/regeneration steps.

Let the intuitive wizard guide your ligand screening

Use the Ligand Screening Wizard for screening and full kinetics (waveRAPID), and choose from guided protocols for two different surface chemistries.

Control ligand level density automatically

The Ligand Screening Wizard works in perfect harmony with the “target level” function, ensuring that ligands are always captured at the same density level for correct data interpretation.

CFCA Wizard

A calibration-free approach to qualification.

Benefit from calibration-free assay

The CFCA Wizard uses the relationship between the diffusion properties of the analyte and the absolute analyte concentration so you don’t need to spend time setting the calibration curve or standard.

Streamline your workflow by starting with crude

With the CFCA Wizard, there’s no need to purify crude samples first – quantify antibodies directly from serum and cell lysate

Quantify active proteins quickly and easily

Since only the analyte binds to the ligand, you can quantify the “active” portion of the sample quickly and easily using the CFCA Wizard.

WAVEdelta combines high sensitivity and signal stability with crude compatibility and rapid throughput.

• GCI label-free technology improves sensitivity and signal stability
  
• No-clog microfluidics work without internal microvalves
• Less device downtime, higher throughput and more accurate results

With WAVEdelta, there’s no need to compromise on quality, speed or experimental setup.

Sensitivity and signal stability

Engineered around our sensitive GCI technology, the WAVEsystem delivers robust kinetic analysis even on low ligand levels. With our temperature-controlled autosampler, you can say goodbye to sample evaporation. Samples are measured using GCI via an expanded sensing field, enabling high-resolution kinetic determination even at very low ligand densities. You can also be confident of accurate results even when measuring long on/off-rates thanks to our low signal drift which translates to a stable signal over time. For your laboratory, it means less waste, and higher resolution for the tight interactions you need in biologics.

No-clog

The innovative design of our patented microfluidic cartridge integrates all the microfluidics into the disposable cartridge instead of within the device core and eliminates clogging. Determine affinity and binding kinetics of crude samples like cell extracts, serum and plasma. With the Creoptix WAVEsystem, you can also analyze virus-like particles, (in)organic nanoparticles, liposomes and nanodisks directly, reliably and easily. Our label-free approach enables you to streamline your experimental protocols and get through more samples in less time. You’ll see your device downtime decrease – and throughput increase.

Throughput

You’re committed to delivering robust scientific results, but you also need to keep an eye on costs and schedules. Our powerful technology is designed to streamline – and speed up – workflows, cut materials and reduce waste. Creoptix waveRAPID is the new way of measuring kinetics that allows you to run more samples and probe more interactions in hours not days. With no need for serial dilutions or DMSO corrections, setup time is significantly reduced, runs are faster, and wells are freed up to run more samples. The faster interaction analysis also means that unstable proteins can also be measured more efficiently. With Creoptix, you naturally work better and faster.

Learn how no-clog microfluidics will cut instrument downtime and increase throughput - watch the video and join Allie the Antibody on a smooth ride through the WAVEsystem.

Overcoming challenges to understand coronavirus

When the novel coronavirus emerged worldwide in January 2020, scientists not only scrambled to develop tests but also to understand the biology of this disease. They began tackling the problem with the tools and knowledge already on hand from studying other infectious diseases like Dengue, Zika, and Ebola. However, SARS-CoV-2 proved challenging in ways current technologies were unable to address. One example is the analysis of molecular interactions between antibodies present in complex matrices like patient blood serum and plasma and SARS-CoV-2 spike protein. Matrix components like serum albumin are often incompatible with optical biosensors due to potential deleterious effects on the microfluidics and high non-specific binding. The WAVEsystem has the potential to overcome these limitations with innovative, disposable, no-clog microfluidics. Using the proprietary GCI technology to deliver high sensitivity, the WAVE provides very high tolerance against crude matrixes and enables the characterization of antibody kinetics directly from clinical blood plasma samples.

Giving hope through antibody-based therapeutics

Monoclonal antibodies are one of the best known classes of biologic drugs, developed to offer high specificity and affinity to their targets. While clinical efficacy is the ultimate outcome, getting there relies on understanding the physiological conditions to which the antibody will be exposed. During drug development, properties such as binding affinity are evaluated alongside other qualities to predict clinical efficacy. Comprehensive characterization includes real-time analysis of binding kinetics through accurate measurement of association and dissociation rates for the antibody-target interaction. To translate these results to clinic, studies should mimic conditions of the native environment as closely as possible. Creoptix allows researchers to study biologic drugs in a wide range of biofluids and other complex matrices.

Developing GPCR therapeutics for metabolic disorders and oncology

G-protein-coupled receptors (GPCRs) comprise a large class of integral membrane proteins. They regulate a variety of cells’ physiological processes and are popular therapeutic targets. Today, more than 30% of available pharmaceuticals target GPCRs in indications such as hypotension, hyperglycemia and cancer. However, membrane proteins are notoriously difficult to study as they become highly unstable following extraction from the cell membrane. Their large size can also be an issue in traditional characterization approaches. With patented no-clog microfluidics, the Creoptix WAVE allows researchers to investigate GPCRs both in solution (detergent-solubilized or reconstituted into a lipidic environment such as nanodiscs) and in membranes, where they can retain their native conformation. Using the proprietary GCI technology to deliver exceptional sensitivity, the Creoptix WAVE can resolve a wide range of affinities and off-rates for enhanced understanding of G-protein/GPCR interactions.