Date recorded: March 22 2011

Duration: 45 minutes 52 seconds

Surface charge can be used as a means of predicting protein aggregation. One common technique for measuring protein charge is electrophoretic light scattering. Historical challenges with this method causing sample aggregation are addressed
Table of contents
1. Measuring Protein Charge – Historical Challenges & Recent Developments
01:54
2. Protein Zeta Potential & Charge Historical Challenges & Recent Developments
00:58
3. Outline
00:40
4. Protein Charge – Increasing Interest
01:30
5. Google Scholar Citations
01:03
6. Importance
02:18
7. High Concentration Biotherapeutics
01:24
8. Pioneers
00:57
9. Protein Charge vs. pH
01:25
10. Not As “Simple” As It Might Seem
01:23
11. Calculating Protein Charge
03:02
12. Calculating Protein Charge – Methodology
00:43
13. Capillary Electrophoresis (CE)
01:22
14. CE Pros, And Cons
02:28
15. CE vs. pH Titration
02:35
16. Membrane Confined Electrophoresis (MCE)
01:02
17. MCE Pros And Cons
01:05
18. Electrophoretic Light Scattering (ELS)
01:41
19. ELS Pros And Cons
01:49
20. Invasive??
01:25
21. Protein ELS Measurements – Guidelines
03:19
22. Solutions Currently Under Development
01:14
23. Protein Denaturation At The Electrodes
00:39
24. Integration Of A Diffusion Barrier*
01:45
25. Discarding The Rules
01:56
26. Example Diffusion Barrier ELS Results
01:12
27. Acknowledgements
00:43
28. Questions?
03:24
29. Contact Information
00:56