Date recorded: August 26 2014

Duration: 43 minutes 51 seconds

The second virial coefficient (A2, B22) has been identified as a potential parameter to assess and optimize formulation stability. Traditionally, the measurement is time consuming in batch mode, since preparation of various concentrations of a sample is necessary with associated time-consuming sample transfer and cuvette cleaning steps. The advent of automated DLS platforms now opens up the possibility of automated static light scattering measurements.This webinar describes the benefit of using an automated sample well-plate device such as the Zetasizer APS for speeding up and simplifying the process of protein formulation screening. Applications examples are shown throughout.

Table of contents
1. Welcome
02:05
2. Speed your way through protein formulation screening by automating your measurements:A2/B22 with the Zetasizer APS
00:56
3. Abstract
00:16
4. Outline
01:05
5. Protein Formulation
02:44
6. Formulation: Aggregation temperature
03:00
7. Formulation: Size change with concentration
04:49
8. Formulation: Intensity with concentration
04:47
9. Value of kD and B22 coefficients
01:47
10. Automated Dynamic Light Scattering
01:48
11. Zetasizer APS
03:22
12. Previously only DLS, now also SLS
01:16
13. Zetasizer Tools – Options – Instrument Type
00:32
14. Tools – Macros – APS Auto SLS
02:05
15. APS Auto SLS details
02:26
16. APS Auto SLS options
00:06
17. Tools – Macros – APS Auto SLS
00:14
18. APS Auto SLS options
01:09
19. Data can be exported
00:40
20. Copy from Record into Excel
00:33
21. Acknowledgements & References
01:59
22. Questions & Contact Information
06:12