Date recorded: February 01 2018

Duration: 46 minutes 30 seconds

Dynamic Light Scattering (DLS), sometimes referred to as Photon Correlation Spectroscopy or Quasi-Elastic Light Scattering, is a technique classically used for measuring the size of particles typically in the sub-micron region, dispersed in a liquid. The sensitivity of some modern systems is such that it can also now be used to measure the size of macromolecules in solution.

In this presentation Dr Mike Kaszuba will summarize how the technique works, what its size and concentration limits are, and discuss its advantages and disadvantages.

Table of contents
1. Welcome
00:14
2. Introduction
00:05
3. Dynamic light scattering in 30 Minutes
00:36
4. Agenda
00:17
5. Agenda
00:03
6. Dynamic Light Scattering and Brownian Motion
01:16
7. Brownian Motion
00:51
8. Hydrodynamic Size
01:51
9. DLS Instrument Components
02:22
10. Intensity Fluctuations and Brownian Motion
00:36
11. Constructive and Destructive Interference
01:47
12. Agenda
00:05
13. Correlation in Dynamic Light Scattering
01:56
14. Correlation
00:45
15. Correlation
01:47
16. Correlation
00:44
17. Correlation
00:29
18. Correlation
00:05
19. Correlation
01:07
20. Correlation
01:05
21. Correlation Functions
00:11
22. Agenda
00:22
23. Correlation Functions
01:09
24. Analysing The Correlation Function
00:05
25. Analysing The Correlation Function
01:07
26. Cumulants Analysis
01:26
27. The z-Average Diameter
00:50
28. Polydispersity Index
01:16
29. Zetasizer Distribution Algorithms
02:51
30. Size Distributions in the Zetasizer Software
01:04
31. Intensity Size Distributions
01:27
32. Volume Size Distributions
01:29
33. Number Size Distributions
00:48
34. Size Distributions From DLS
00:48
35. Volume/Number Distributions: Recommended Use
00:52
36. Any Questions?
12:44