Case study: Using NTA to study nanoparticle diffusion

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00:00:00 Welcome!
00:01:22 Using NTA to study nanoparticle diffusion
00:01:56 NanoSight’s Technology
00:02:55 Nanoparticle Tracking Analysis
00:03:26 Principle of measurements
00:03:58 NTA instruments
00:04:20 Information
00:05:20 Light scattering vs fluorescence mode
00:06:36 Diffusion of nanomaterials in liquid media
00:09:12 Importance of diffusion in drug delivery
00:11:14 Synthesis of functionalised silica nanoparticles
00:11:31 Thiolated silica nanoparticles as a model system
00:12:37 DLS and TEM
00:14:12 Surface functionalisation
00:15:40 Sizes of nanoparticles before (1) and after PEGylation (2-4)
00:16:45 Small angle neutron scattering
00:17:22 Diffusion of functionalised silica in polymer solutions
00:17:28 Diffusion of thiolated silica in polymer solutions
00:19:55 Diffusion of PEGylated silica in polymer solutions
00:21:07 Diffusion of thiolated and PEGylated nanoparticles in PAA solutions of different pH
00:23:17 Diffusion of PEGylated nanoparticles in PAA and PEG
00:24:28 Diffusion of functionalised silica in mucin dispersions and mucus gels
00:25:24 Mucosal adhesion and penetration of nanoparticles
00:25:57 Diffusion of nanoparticles in porcine gastric mucin dispersions
00:26:59 Diffusion coefficients at different temperatures
00:27:35 Permeation of nanoparticles through freshly excised porcine gastric mucosa
00:29:38 Nanoparticles functionalised with different alkyne terminated poly(2-alkyl-2-oxazolines)
00:30:11 Characterisation
00:30:48 Diffusion in mucus studied using NTA
00:32:02 Ex vivo mucosa penetration
00:33:04 Ex vivo mucosal penetration
00:33:37 Effect of Particle Surface Chemistry on Mucosal Penetration
00:34:26 Conclusions
00:35:31 Acknowledgements
00:37:32 Thank you for your attentionAny questions?
00:43:27 Contact Information and Thank You
• Nanoparticle Tracking Analysis (NTA) was used to study the diffusion of silica nanoparticles functionalized with different polymers in aqueous solutions of water-soluble polymers and in dispersions of porcine gastric mucin
• It was found that the diffusivity of nanoparticles is affected by their dimensions, medium viscosity, and specific interactions between nanoparticles and macromolecules in solution. Strong attractive interactions such as hydrogen bonding were found to hamper diffusion.
• This study demonstrated that decoration of nanoparticle surfaces with poly(2-ethyl-2-oxazoline) or poly(ethylene glycol) enhances their ability to travel through mucus. The diffusion results generated by NTA were in good agreement with the data on the permeation of these nanoparticles through ex vivo porcine stomach mucus.