Adeno-Associated Viral Titer in minutes, using Nanoparticle Tracking Analysis (NTA)

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00:00:00 Welcome!
00:01:04 Contents
00:01:48 NanoSight Models
00:02:45 Adeno Virus (Directly From Serum)
00:04:36 Influenza Virus
00:05:38 Lentivirus
00:06:43 Small Viruses (35-45 nm)
00:07:42 Adeno Associated Virus (AAV)
00:11:06 AAV Titer
00:11:06 AAV Aggression State: Thermal Stress Test
00:12:44 AAV Electrostatic Gold Labeling Approach
00:13:36 Electrostatic vs. Antibody approach
00:14:53 TEM Analysis
00:14:53 Optical Arrangement for NanoSight Instruments
00:15:53 Direct Visualization of Nanoparticles in Suspension
00:15:53 Sizing: Stokes-Einstein
00:17:23 Concentration: Particles are Counted by Number
00:17:23 Fluorescent Mode
00:18:46 Analysis of 100 nm Fluorescence Standard Particles Suspended in FBS
00:18:46 Summary
00:20:22 Thank you for your attentionAny questions?
00:24:17 Contact Information

• Nanoparticle Tracking Analysis (NTA) Characterization of Gold-Labeled Adeno Associated Virus (AAV) and Other Small Viruses 

• NTA is an analytical tool that measures the size and concentration of virus particles. 

• NTA can measure virus titer in about 5 minutes for viruses above Dh ~35 nm. 

• AAV (Dh ~ 25 nm) is too small and does not scatter enough light to be measured directly by NTA. 

• Gold-labeled AAV enhances the light scattering and enables NTA characterization to rapidly measure AAV particle concentration and aggregation state.