Enzyme kinetics assays with MicroCal ITC systems

Isothermal Titration Calorimetry (ITC) is a powerful and versatile technique that is widely used for measuring the binding affinity and thermodynamics of equilibrium association reactions. ITC is also an accepted, universal label-free assay technique that can be applied to any enzyme-catalyzed system, provided that the reaction is associated with a change in enthalpy – see the companion whitepaper and other review articles [1-8]. ITC does not rely on a spectroscopic readout and labelled reagents, and the experiments are quick – ITC data for a Michaelis-Menten curve can be generated in 30 -90 minutes with current instrumentation – and only small amounts of material are required, making it an appealing alternative for enzyme kinetics and inhibition assays.

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