Date recorded: May 14 2020
We have developed a fast, convenient and precise methodology using isothermal titration calorimetry (ITC) to elucidate the enzymatic properties of the soluble epoxide hydrolase (sEH) and characterise its inhibition. sEH catalyses epoxy lipid hydrolysis and is implicated in cardiovascular diseases, diabetes and pain.
The ITC-based single injection methodology (SIM) is based on measuring the heat generated in an enzymatic reaction, a feature that is common to every catalyzed process. It has therefore the potential to be applied to many biological systems that cannot be readily followed by other enzyme assay techniques.
In this webinar we will describe the method, present the data generated and discuss any troubleshooting and optimization that was required.
Our results have been recently published: G. Abis, R. Pacheco-Gómez, T. T. T. Bui, and M. R. Conte, “Isothermal Titration Calorimetry Enables Rapid Characterization of Enzyme Kinetics and Inhibition for the Human Soluble Epoxide Hydrolase,” Analytical Chemistry, volume 91, pages 14865-14872, 2019, (link https://europepmc.org/article/med/31660733) and reported on King’s College London News Centre: