Research insights into biomolecular interactions
The MicroCal VP-ITC is a powerful and sensitive, easy-to-use isothermal titration calorimeter that offers the ability to investigate any biomolecular interactions. This fast, convenient, label free and in-solution technique delivers the information needed to characterize the molecular interactions of proteins, antibodies, nucleic acids and other biomolecules. The system is widely used in research environments for lead optimization, hit validation, assessing the driving mechanisms of binding and studying enzyme kinetics and inhibitor design.
The MicroCal VP-ITC delivers affinity data that provide insight into biomolecular interactions and can be used to investigate any such interaction. Isothermal Titration Calorimetry is label free, and in solution, and is fast, convenient and offers a free choice of buffers and no limitations with respect to molecular weight.
The MicroCal VP-ITC is complete system that requires no additional accessories, reagents or consumables. Once the samples are loaded it operates unattended, freeing the user for other tasks.
Isothermal Titration Calorimetry (ITC) measures the binding affinity and thermodynamics between any two biomolecules. When substances bind, heat is either released or absorbed. Measurement of this heat change in an ITC experiment enables accurate determination of binding constants (KD), interaction stoichiometry (n), enthalpy (ΔH) and entropy (ΔS), providing a complete thermodynamic profile of the molecular interaction in a single experiment.
MicroCal VP-ITC can also be used to determine heat capacity changes upon binding by performing experiments over a range of temperatures.
- Directly measures millimolar to nanomolar affinities (KDS) (10-2 to 10-9 M).
- Measures nanomolar to picomolar disassociation constants using competitive binding techniques (109 to 1012 M).
- Nonreactive Hastelloy cells for excellent chemical resistance and applicability with biological samples.
- Fixed-in-place cells for reproducible, ultrasensitive performance with low maintenance.
- Precision liquid delivery system for accurate and reproducible injections.
- Coin shaped cell optimizes sample mixing.
- Three user-selectable response times for application versatility.
- User-selectable mixing speeds to match sample conditions.
- Peltier controlled for rapid temperature equilibration.
- Includes ThermoVac™ sample preparation and cleaning device.
- Controlled by VPViewer™ software and data analysis performed with Origin™.
- True in-solution technique: No labeling or immobilization required. No buffer restrictions. Easily handles turbid solutions.
The MicroCal VP-ITC uses the technique of Isothermal Titration Calorimetry to measure the binding affinity and thermodynamic parameters involved in a variety of molecular interactions.
Principles of Isothermal Titration Calorimetry (ITC)
Isothermal titration calorimeters measure the heat change that occurs when two molecules interact. Heat is released or absorbed as a result of the redistribution and formation of non-covalent bonds when the interacting molecules go from the free to the bound state. ITC monitors these heat changes by measuring the differential power, applied to the cell heaters, required to maintain zero temperature difference between the reference and sample cells as the binding partners are mixed.
Schematic representation of the essential components of an ITC system
The reference cell is usually filled with water. The sample cell contains one binding partner and a stirring syringe which holds the ligand. The ligand is injected into the sample cell, in small aliquots, until the ligand concentration is 2-3 times greater than the sample. Each injection of ligand results in a heat pulse that is integrated with respect to time and normalized for concentration to generate a titration curve of kcal/mol vs molar ratio (ligand/sample). The resulting isotherm is fitted to a binding model to generate the affinity (KD), stoichiometry (N) and enthalpy of interaction (ΔH).
Visit the technology page for more detailed explanation.
- Measurement type:
- Affinity (KD)
- Measurement type:
- Enthalpy ∆H
- Measurement type:
- Entropy ∆S
- Measurement type:
- Stoichiometry (n)
- Sample volume:
- Cell volume:
- Injection syringe volume:
- Sample capacity:
- 4-8 per 8h day
- Cell material:
- 0.5 ncal/s
- Temperature range:
- 2°C to 80°C
- Response time:
- 20s (high feedback)
- Multiple feedback modes:
- Yes (passive, high gain, low gain)
- 100-240V, 50/60Hz, 120W
11.5 kg (Calorimeter)
9 kg (Controller)
- Dimensions (W, D, H):
200mm x 370mm x 440mm (Calorimeter)
150mm x 410mm x 360mm (Controller)
- Temperature (operating):
- 10°C to 28°C
- 0% to 70% RH, non condensing
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