Instead of relying on a titration series, waveRAPID (Repeated Analyte Pulses of Increasing Duration) injects a single concentration, pulsing the sample over the sensing surface at increasing durations, meaning kinetics can be derived from a single well.
How it works
Workflow-based design offering both flexibility and functionality
Every step from assay setup over data evaluation to report writing is simplified with an intuitive design that mirrors the way you work. Our latest release, WAVEcontrol 4.0, also includes Direct Kinetics, a fast, automated and objective way to analyze data.
Assay setup to data reporting in 4 steps
- Design your experiment
Smart design for your experiment before you begin, with our built-in optimizer to simulate data
- Set up your experiment
Use our wizards for a fast and efficient experimental set-up or manually edit for full flexibility
- Evaluate your data
Apply our predefined models to evaluate the results of your experiments. Adjustments can be fully fine-tuned. Evaluate your data with traditional (global) fit or let Direct Kinetics evaluate the data for you
- Report your data
Get access to raw data and export your results in a customizable PDF or Word format with just a few clicks
Data evaluation: Global fitting
Global fitting is the most commonly used data evaluation function in real-time biomolecular interaction analysis. Based on non-linear least-squares fitting, this method finds rates and affinity constants to fit curves as close as possible to the actual data.
Global fitting needs human intervention for obtaining the best results and is driven by “what looks good”. A well-trained scientist would need 3-5 minutes to analyze one interaction.
Data evaluation: Evidence-based estimation
We focus on the evidence supported by your biomolecular interaction data instead. With our brand new Direct Kinetics tool, we rely on robust statistical estimators to deliver an automated calculation of your kinetic parameters with mathematically sound error analysis.
With Direct Kinetics, instead of optimizing the visuals, the error on the determined parameters is minimized. Dozens of interactions are automatically analyzed in 3-5 minutes.
Benefits of WAVEcontrol
- New wizards
pH scouting, support for new WAVEchip® types, and new wizards to improve time-to-result
- Smoother Setup
Copy/paste and import from Excel files for large screens. Drag and drop for full flexibility
- Improved Evaluation
More models for regeneration-free kinetics and automated evaluation with Direct Kinetics (1-click evaluation tool)
- Save time and costs
Benefit from waveRAPID®, a novel method allowing you to obtain kinetics of an interaction by injections from a single well
Our 1-click evaluation tool for more models for regeneration-free kinetics and automated evaluation
With our Direct Kinetics tool, we rely on robust statistical estimators to deliver an automated calculation of your kinetic parameters with mathematically sound error analysis.