Global fitting is the most commonly used data evaluation function in real time biomolecular interaction analysis. Based on non-linear least-squares fitting, this method finds rates and affinity constants to fit curves as close as possible to the actual data.
Global fitting needs human intervention for obtaining the best results and is driven by "what looks good". A well-trained scientist would need 3-5 minutes to analyze one interaction.
With the Creoptix® WAVEdelta’s software evaluation tool Direct Kinetics, instead of optimizing the visuals, the error on the determined parameters is minimized. We focus on the evidence supported by your biomolecular interaction data. We rely on robust statistical estimators to deliver an automated calculation of your kinetic parameters with mathematically sound error analysis. Dozens of interactions are automatically analyzed in 3-5 minutes.
- Quick decision making: rapid computation of confidence intervals to speed up the decision making process
- Fewer errors: minimized error on your kinetic rates by automated, evidence-based estimation for all your data with one click
- Increased transparency: make decisions based on accurate computation of the measurement error
- Reproducible data: mitigate the risk of over-fitting artifacts in your data for increased reproducibility