The Kinetics Guide | Binding kinetics with the WAVE system. Download now

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Creoptix WAVEcore

Next-generation bioanalytical instruments for drug discovery and life sciences for both industry and academic research

The Creoptix® WAVEsystem puts a breakthrough level of binding kinetics analysis at your fingertips by pushing the boundaries of affinity range and sample compatibility. The WAVE's exceptionally high data quality, sample compatibility and automated software facilitates drug discovery and enable new inroads into R&D.

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Key features

The Creoptix® WAVEsystem combines the unrivaled high sensitivity of the GCI in the WAVEcore with the WAVEcontrol software to give you superior data analysis and the WAVEchips, our no-clog microfluidic cartridges, to support a wide range of sample types and sizes. The WAVEsystem comes with a temperature-controlled autosampler that fits vials, two 96- or one 384-well plates for further ease of use. In its entirety, the system gives you an unparalleled platform for real-time label-free binding kinetics.

  • Crude samples, harsh chemicals, and particles up to 1000 nm
  • Self-contained disposable cartridge and small footprint
  • Ultra-fast transition times of 150 ms with reliable determination of off-rates up to 10 s-1
  • Superior signal-to-noise ratios (0.01 pg/mm2 at 1 Hz)
  • Reliable kinetics and binding affinities (KD) from low pM to low mM with from signals below 1 pg/mm2
  • Analyze large ligand‑to‑analyte molecular weight ratios up to >1000:1
  • Up to 120 hours of unattended operation

Advantages

The Creoptix® WAVEsystem combines crude sample robustness, high sensitivity, and sophisticated software, to create a novel optical biosensor platform for real-time label-free binding kinetics. Engineered around innovative microfluidics and our proprietary Grating-Coupled Interferometry (GCI) technology, novel data from difficult samples is now possible for unsurpassed insight.

Label-free data like you've never seen before

The WAVE houses our patented Grating-Coupled Interferometry (GCI) technology. Together with a temperature-controlled autosampler that can handle 2x 48-vial racks, 96- or 384-well plates or combinations thereof, the WAVE offers the sensitivity to work with low immobilization levels and large ligand-to-analyte molecular weight (MW) ratios.

High sensitivity

GCI differs from SPR in how the refractive index change is readout. In SPR, the surface plasmon is quickly attenuated and can pick up signals only from a few interactions. In GCI, each photon travels through the whole waveguide, and hence can pick up the signals from much more interactions, resulting in an intrinsically higher sensitivity.

In addition, the waveguide’s evanescent field penetrates less into the bulk, minimizing disturbances caused by bulk refractive index changes. Refractive index changes at the sensor surface are measured as time-dependent phase-shift signals. This allows for superior high signal-to-noise ratios, measurement of kinetics (ka, kd) and affinity (KD) from low pM to low mM, from signals below 1 pg/mm2 (equivalent to <1RU), for higher sensitivity and confident kinetic analysis.

[wavecore2.jpg] wavecore2.jpg

Download the WAVEcore brochure.

Large Ligand-to-Analyte Molecular Weight Ratios

Sensitivity is often limiting for accurate and reliable kinetic analysis of molecular interactions between large drug targets and small molecule inhibitors. Large target-to-analyte molecular weight ratios present a big challenge for traditional label-free interaction analysis and can significantly impact data quality. The Creoptix® WAVE is compatible with the high ligand-to-analyte molecular weight ratios, providing outstanding resolution and reliable kinetics with at low immobilization levels for target-to-analyte molecular weight ratios for up to >1000:1. The outcome is increased sensitivity to accurately measure low-potency small molecules or fragments, or targets with low activity.

Read the technical note: Sensitive kinetic analysis of small molecules binding to large drug targets

More Signal, Less Noise

GCI produces an evanescent field that penetrates less deep into the bulk than SPR, minimizing the disturbance caused by bulk refractive index changes. This reduction in noise leads to superior signal-to-noise ratios.

[wave core 3.png] wave core 3.png

Robust Readout

Our patented GCI technology creates an interference readout in the time-domain and within the waveguide, instead of a space-domain signal being projected onto a CCD camera used with traditional waveguide interferometry methods. Measuring refractive index changes on the sensor surface as time-resolved phase-shift signals provides a more robust readout regardless of temperature drifts or vibrations, translating to superior resolution in signal and time.

Explore the Creoptix WAVEsystem

WAVEchip

WAVEchip®- Innovative design and patented microfluidic cartridge to support crude samples, pathogenic samples, harsh solvents, and large particles up to 1000 nm normally only achieved with plate-based assays for kinetic analysis not possible before.

WAVEchip

WAVEcontrol

Move seamlessly from sample to data in a simple stepwise process, generating outputs at the touch of a button. Benefit from waveRAPID, a novel method implemented on the existing WAVEdelta instruments allowing you to obtain kinetics of interaction by injections from a single well.

WAVEcontrol

WAVEcare

Carefree or basic support for high-quality kinetic data and unparalleled performance.

WAVEcare

Specification

The Creoptix WAVE puts a breakthrough level of kinetics analysis at your fingertips by pushing the boundaries of affinity range and sample compatibility. The system’s exceptionally high data quality, sample compatibility and automated software facilitates drug discovery and enable new inroads into R&D.

Download the Creoptix WAVEcore specification and the Creoptix WAVEdelta specification here.

General

SpecificationWAVEWAVEdelta
Noise (RMS)<0.01 pg/mm2 @ 1 Hz<0.01 pg/mm2 @ 1 Hz
Drift<0.3 pg/mm2/min<0.3 pg/mm2/min
Readout Frequency1 Hz, 10 Hz or 40 Hz1 Hz, 10 Hz or 40 Hz
Association Const. Rangeka = 103 – 5x107 M-1 s–1 (small molecules)
ka = 103 – 3x109 M-1 s–1 (large molecules)
ka = 103 – 5x107 M-1 s–1 (small molecules)
ka = 103 – 3x109 M-1 s–1 (large molecules)
Dissociation Const. Rangekd = 10–5 – 10 s–1kd = 10–5 – 10 s–1
Analysis Temperature Range15 °C – 40 °C4 °C – 45 °C
(max 20 °C below ambient)
Molecular Weight LimitNo lower limitNo lower limit
waveRAPID® FunctionalityNoYes

Fluidics

SpecificationWAVEWAVEdelta
Flow Channels / Path2, parallel4, parallel
Channel Referencing1-4 and 4-1 or 2-3 and 3-2Any combination of the 4 channels
Flow CellsSealed, disposable, integrated into disposable WAVEchipSealed, disposable, integrated into disposable WAVEchip
Flow Rate1 – 400 µl/min1 – 400 µl/min
Crude Sample RobustnessYesYes

Sample Handling

SpecificationWAVEWAVEdelta
Sample Capacity2x microtiter plates (96 or 384 well, standard or deep well) or vial racks (48 positions of 1.5ml)2x microtiter plates (96 or 384 well, standard or deep well) or vial racks (48 positions of 1.5ml)
Buffer1 bufferAutomatic switching between 4 buffers
DegasserBuilt-inBuilt-in
Injection Volume< 450 µl, 100 µl typical< 450 µl, 100 µl typical
Sample Volume RequiredInjection volume plus 15-50 µl (application dependent)Injection volume plus 15-50 µl (application dependent)
Sample Storage TemperatureAmbient or 4°C – 20°C regulatedAmbient or 4°C – 20°C regulated
Sample RecoveryYesYes
Automation120h of unattended operation120h of unattended operation

Data Treatment

SpecificationWAVEWAVEdelta
Information ProvidedKinetic and affnity data (ka, kd, KD)Kinetic and affnity data (ka, kd, KD)
GraphsReal-time curves, multiple curve overlays, fit, report point plotsReal-time curves, multiple curve overlays, fit, report point plots
Data ExtractionCurves, ka, kd, KD tables, graphs, reportsCurves, ka, kd, KD tables, graphs, reports
Data AnalysisFully automated data evaluationFully automated data evaluation
Kinetic ModelsPredefined models including 1:1 interaction, mass transport, heterogenous ligand, conformational change and bivalentPredefined models including 1:1 interaction, mass transport, heterogenous ligand, conformational change and bivalent
Direct KineticsYesYes

Support

Protect your investment and ensure optimized performance at all times with Malvern Panalytical's service plans. When you purchase a Malvern Panalytical product we understand that this is just the first stage of a working relationship that will last for the lifetime of the instrument. Depending on your needs, Malvern Panalytical will provide support for your business. We offer the following service options:

Carefree Program

Basic program

Ideal for the regular user 

Know what you are up to and plan your maintenance budget ahead of time

Ideal for the occasional user 

Call us when you need us the most for a PM visit or a repair


The first twelve months

Care for the first year of your Creoptix WAVE is on us, with a warranty period that covers travel, labor and parts costs for one (1) onsite preventive maintenance (PM) visit to be carried out within 12 months. Travel, labor and parts costs for unscheduled repairs are also included. The different support programs ensure the continuous, high performance operation of your WAVE.

Learn more here or download our WAVEcare brochure here.

Robust with multiple applications. Dependable results with high sensitivity.

James Schouten — Mologic