OMNISEC REVEAL

The ultimate in integrated advanced detection for GPC/SEC

Molecular weight, intrinsic viscosity and numerous other parameters can be measured with OMNISEC REVEAL, the world’s most advanced integrated GPC/SEC multi-detector available. It can be added to any existing conventional GPC/SEC system or used in conjunction with OMNISEC RESOLVE to form a complete GPC/SEC solution, to offer comprehensive molecular characterization of synthetic and natural polymers, as well as proteins. 

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Overview

OMNISEC REVEAL can be used to relate a polymer’s molecular properties to its bulk characteristics in a wide range of applications, from the packaging and paint industries to the manufacturing of food additives and pharmaceuticals. Its protein characterization capabilities can be used to study the effects of oligomerization, aggregation and conjugation on activity and efficacy in academic research and biopharmaceutical development.

The ultimate in advanced detection for GPC/SEC

  • Complete flexibility in detector configuration. Only purchase the detectors which add value to you now, with the security that any detector you require in the future can be installed onsite

Multi-detection SEC offers unparalleled information on the characterization of polymers, polysaccharides and proteins

  • Measuring absolute molecular weight, intrinsic viscosity, hydrodynamic radius, Mark-Houwink parameters, radius of gyration, composition, conformation and more
  • Rely on OMNISEC to help you understand, control and optimize your product performance
  • Detection and quantification of protein aggregation to drive faster sample characterization and development

Fully integrated temperature-controlled detector module for improved detector stability and sample characterization

  • Improve separations and result accuracy with the column oven and temperature-controlled integrated detector compartment – all installed as standard! 
  • Minimized inter-detector tubing, maintained at the same temperature as the detector, reduces band broadening effects to further increase signal quality

More reliable measurements with market leading light scattering sensitivity

Measure low concentration samples with a highly sensitive and stable refractive index detector

  • Measure molecular weight of polymers as low as 200 g/mol!
  • Injection masses as low as 100 ng for characterization of samples which were previously unmeasurable using advanced detection
  • Make sure you don’t miss low molecular weight and low concentration contaminants or protein fragments 

Improved structural comparisons enabled with the unique mechanical auto balance of the differential viscometer

  • Boost lab productivity and reduce service costs with a novel self-balancing viscometer design with user exchangeable capillaries

See the full range of your samples UV absorbance with the UV-PDA

  • Distinguish chemical changes by measuring absorbance across the full UV/Vis spectrum (190-900nm) using the photodiode array
  • All UV channels are recorded giving you the flexibility to choose what wavelength to use in post-processing

All systems can be fully serviced and supported onsite

  • No need to worry about shipping systems for service or support, all units can be fully serviced onsite to ensure minimum downtime

How it works

Following separation of the sample according to its hydrodynamic volume the sample will pass through 1 or more detectors where its presence is recorded. OMNISEC REVEAL is a fully integrated multi-detection module which can contain up to 4 different detectors. 

The RI and UV detectors give information about the concentration of the components in the sample. The light scattering detectors respond to the intensity of light scattered by the sample which is related to molecular weight and also allows Rg of large molecules to be calculated. The viscometer measures the changing solution viscosity to calculate the intrinsic viscosity of the sample which is a measure of structure. 

Coupling the efficient sample separation of OMNISEC RESOLVE with the fully optimized, multi-detector, information-rich analysis delivered by OMNISEC REVEAL, Malvern Panalytical has produced a market-leading solution for researchers and formulators developing novel natural and synthetic polymers, pharmaceutical and biopharmaceutical products and foods.

05_RI

Refractive Index

  • Measure the concentration of any solute with excellent sensitivity
06_UVvisPDA

UV/Vis PDA

  • Measure absorbance at any wavelength from 190-900 nm
  • Assess changes in absorbance spectra
  • Characterize copolymers
07_LightScattering

Light Scattering

  • Measure absolute molecular weight in a wider range of applications
  • Measure as little as 100 ng of material
  • Measure molecular weights down to 200 Da
08_Viscometer

Viscometer

  • Better intrinsic viscosity measurements thanks to automated balancing
  • Reduced service cost and time thanks to user-exchangeable capillaries

Specification

System

Dimensions (W, D, H) 420, 640, 600 mm
Weight 40 kg
Detector temperature range 20 - 65 °C
Software OMNISEC software v10 (or later)
Data acquisition rate 100 Hz
Patents

US20140060162A1 & EP2619543B1 "Modular capillary bridge viscometer"

US20140144214A1 & EP2619544A1 "Automatically balanced capillary bridge viscometer"

Detector 1

Detector Light scattering
Measurement principle RALS 90° angle, LALS 7° angle
Light source 50 mW, 640 nm laser
Cell volume 18 µL
Dynamic range 2500 mV
Baseline noise <0.1 mV
Baseline drift <0.2 mV/hr

Detector 2

Detector Differential refractive index
Measurement principle Deflection
Cell volume 12 µL
Dynamic range ±2.5 x 10-4 RIU
Baseline noise <10-7 RIU
Baseline drift <3 x 10-7 RIU/hr

Detector 3

Detector Viscometer
Measurement principle 4-capillary Wheatstone bridge with self-balancing mechanism and user-exchangeable capillaries
Detector volume 17 µL/capillary
Differential pressure dynamic range ±100 kPa
Differential pressure baseline noise <1 Pa
Inlet pressure dynamic range 100 kPa
Inlet pressure baseline noise 0.01 kPa
Baseline drift <0.2 kPa/hr

Detector 4

Detector Diode-array-based UV/Vis spectrometer
Wavelength 190 - 900 nm
Number of wavelengths 1024
Wavelength accuracy <1 nm
Wavelength resolution 0.6 nm
Cell volume 7.5 µL
Path length 10 mm
Baseline noise 2 x 10-5 AU
Baseline drift 5 x 10-4 AU/hr

Measurement type 1

Measurement type Absolute molecular weight
Measurement range 200 - >107 g/mol
Minimum quantifiable mass Using HPLC-SEC:
100 ng of 100 kDa molecular weight polystyrene in THF
100 ng of BSA in PBS
Measurement principle Light scattering (LALS or RALS)

Measurement type 2

Measurement type Intrinsic viscosity
Minimum quantifiable mass Using HPLC-SEC:
3 µg of 100 kDa molecular weight polystyrene in THF
Measurement principle 4-capillary Wheatstone bridge

Measurement type 3

Measurement type Concentration
Minimum quantifiable mass
Using HPLC-SEC:
100 ng of 100 kDa molecular weight polystyrene in THF
100 ng of BSA in PBS
Measurement principle Differential refractive index detection

Operating environment

Temperature 15 °C - 30 °C