記録された日時: November 20 2018

Duration: 01 hours 21 minutes 41 seconds

Dynamic light scattering (DLS) is a common laboratory technique routinely used for sizing proteins, nanoparticles, polymers, & colloidal emulsions. By utilizing the improved accuracy and resolution of multi-angle dynamic light scattering (MADLS®), it is now possible to determine the absolute particle concentration distribution using the Zetasizer Ultra. This provides the user with a fast assessment of the concentration of each population of particles present within their dispersion.  In this webinar we will give an overview of the particle concentration analysis on the Zetasizer Ultra and provide some examples for liposomes and virus particles.
Table of contents
1. Enhanced Precision Multi Angle DLS
01:06
2. Poll Question
00:00
3. Enhanced Precision Multi Angle DLS
02:41
4. Poll Answers
00:00
5. Enhanced Precision Multi Angle DLS
01:01
6. Brownian motion
01:59
7. Dynamic light scattering (DLS)
00:42
8. Brownian motion
00:08
9. Dynamic light scattering (DLS)
01:25
10. Correlogram deconvolution
01:00
11. Particle Size Distribution
01:03
12. Intensity to mass transform
01:59
13. DLS Results
01:19
14. Limitations – Experimental Noise
03:01
15. Intensity to mass transform
01:01
16. Limitations – Mie Scattering effects
04:26
17. Inherent Polydispersity
01:23
18. DLS Precision – Resolution Limits
02:34
19. Compounding the uncertainty
01:23
20. Intensity to mass transform
00:18
21. Compounding the uncertainty
02:04
22. Historical solution
02:00
23. Historical Solution – Ideal Samples
02:21
24. Historical solution – real samples
01:51
25. The challenge
01:30
26. The Solution
04:04
27. Adaptive Correlation
03:24
28. Multi Angle correlogram collection
01:43
29. enhanced Resolution Fitting
01:10
30. Enhanced precision size distribution
01:23
31. MADLS Example – real samples
04:05
32. MADLS – Methodology
01:52
33. Plus – particle concentration
00:42
34. Particle concentration
01:34
35. concentration – AAVs
01:10
36. Concentration – Nanoparticles
01:07
37. Particle concentration – limitations
04:21
38. Particle concentration
02:58
39. Particle concentration – range
01:52
40. Thank you for your attention
00:10
41. Question & Answer SessionAsk your question by typing within the Q & A chat facility Listening on-demand ?Send your questions to:events@malvernpanalytical..co
01:44
42. Particle concentration – limitations
03:16
43. Question & Answer SessionAsk your question by typing within the Q & A chat facility Listening on-demand ?Send your questions to:events@malvernpanalytical..co
06:51