Date recorded: April 30 2015

Duration: 01 hours 01 minutes 25 seconds

Watch our webinar on advanced Isothermal Titration Calorimetry (ITC) data analysis with guest presenters from ITC analysis software providers, AFFINImeter. Together we show how the latest advances in the field of ITC data analysis enable users to “squeeze” the ITC isotherm(s) to get more information than just thermodynamic data and to expand the range of applications of ITC. This webinar is very helpful for ITC users studying complex interactions, who are having difficulties with their data analysis, or simply want to take their analysis to the next level. It is also relevant for researchers from academia and industry working in biomedical applications, particularly in the area of drug design.

Table of contents
1. Welcome
02:12
2. How to Gain Insights into Complex Modes of Interaction with ITC
01:08
3. Overview
00:53
4. Isothermal Titration Calorimetry: Standard Models vs. Nonstandard Models
00:03
5. ITC
01:04
6. ITC
01:49
7. ITC's Black Legend
01:01
8. Standard Model Example
01:25
9. Standard Model Example 2
01:09
10. Nonstandard Model Example
00:50
11. Nonstandard Model Example 2
00:51
12. Nonstandard Model Example
00:41
13. Nonstandard Model Example 2
00:45
14. Nonstandard Model: Homotropy
01:34
15. Nonstandard Model: Homotropy
00:52
16. Nonstandard Model: Homotropy
00:33
17. Nonstandard Model: Homotropy
02:06
18. Nonstandard Model: Homotropy
00:51
19. Nonstandard Model: Homotropy
00:58
20. Nonstandard Model: Homotropy
02:02
21. Nonstandard Model: Homotropy
00:39
22. Nonstandard Model: Heterotropy
01:13
23. Nonstandard Model: Heterotropy
01:15
24. Nonstandard Model: Heterotropy
01:23
25. Competitive Binding
01:26
26. Cooperative Binding
01:12
27. Analytical Tools to Gain Insights into Complex Modes of Interactions with ITC
00:32
28. Heterogeneous Mixtures of Ligands
01:55
29. In the Mix: Simultaneous Affinity
00:21
30. Direct Titration
02:42
31. The Reaction Builder
00:06
32. Reaction Builder Example
00:29
33. Reaction Builder Example 2
00:09
34. New Model
00:16
35. New Model
00:16
36. New Model
00:31
37. ITC Isotherms
02:31
38. Global Fitting
01:16
39. Global Fitting Results
00:24
40. The Species Distribution Plot
02:17
41. Heterogeneous Mixtures of Ligands
00:44
42. Heterogeneous Mixtures of Ligands
00:25
43. Experimental Setup
02:13
44. Application in the Pharmaceutical Industry
01:17
45. Multiple Site Ligand Binding
01:22
46. Multiple Site Ligand Binding
01:02
47. Multiple Site Ligand Binding
00:43
48. Are S1 and S2 of CaM Independent?
01:10
49. Are S1 and S2 of CaM Independent?
00:32
50. Global Fitting
01:01
51. How to Gain Insights into Complex Modes of Interaction with ITC?
01:00
52. Thank you for your attentionAny questions?Please type your questions in using the Q&A panel on the right side of your screen
00:18
53. Contact Information
05:58
54. Find out more
00:00