Features and benefits

MicroCal PEAQ-ITC is designed for ease-of-use and exceptional sensitivity. The system directly measures heat released or absorbed during biochemical binding events, from which it calculates binding affinity (KD), stoichiometry (n), enthalpy (ΔH), and entropy (ΔS). With minimal sample preparation and system optimization data is generated quickly and easily. The wide affinity range enables analysis of weak to high affinity binders, with excellent reproducibility. MicroCal ITC is an essential tool for any research laboratory studying biomolecular interactions where high sensitivity and fast results are paramount.

  • User-friendly guided workflows with embedded help videos give any level of user the ability to generate high quality data.
  • High signal to noise gives more confidence in accessing data quality and relevance of generated affinity and thermodynamic parameters.
  • Automated washing with detergent of the sample cell and titration syringe assists in producing high quality reproducible data.
  • All binding parameters (affinity, stoichiometry, enthalpy and entropy) in a single experiment.
  • Quick to first results with no assay development, no labeling, no immobilization and no molecular weight limitations.
  • Sensitivity to investigate any biomolecular interaction using as little as 10µg of protein.
  • Directly measures millimolar to nanomolar affinities (KDS) (10-2 to 10-9 M).
  • Measures nanomolar to picomolar disassociation constants using competitive binding techniques (10-9 to 10-12M).
  • Nonreactive Hastelloy for chemical resistance and compatibility with biological samples.
  • Compatible with non-aqueous solvents.
  • Upgradable to fully automated MicroCal PEAQ-ITC Automated.

A wide range of applications can be investigated with MicroCal PEAQ-ITC, including characterization of molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules. It can also be used to measure enzyme kinetics. 

MicroCal PEAQ-ITC analysis software offers experiment design simulation, batch evaluation of large data sets, automated assessment of data quality and a streamlined user interface that guides the user to final figures and presentation quality graphs quickly and easily.

  • Open multiple experiments in a single session.
  • Supports many automated fitting models.
    • Dissociation.
    • Enzyme Kinetics - Multiple Injections.
    • Enzyme Kinetics - Single Injection.
    • One Set of Sites.
    • Sequential Binding Sites.
    • One Set of Sites - SIM.
    • Two Sets of Sites.
    • Competitive.
  • Automated assessment of data quality.
    • Good quality data – Binding.
    • Good quality data - No binding.
    • Poor quality data – Check data.

How it works

Isothermal titration microcalorimeters measure the heat change that occurs when two molecules interact. Heat is released or absorbed as a result of the redistribution and formation of non-covalent bonds when the interacting molecules go from the free to the bound state. ITC monitors these heat changes by measuring the differential power, applied to the cell heaters, required to maintain zero temperature difference between the reference and sample cells as the binding partners are mixed. The reference cell usually contains water, while the sample cell contains one of the binding partners (the sample, often but not necessarily a macromolecule) and a stirring syringe which holds the other binding partner (the ligand). The ligand is injected into the sample cell, typically in 0.5 to 2 μL aliquots, until the ligand concentration is two- to three-fold greater than the sample. Each injection of ligand results in a heat pulse that is integrated with respect to time and normalized for concentration to generate a titration curve of kcal/mol vs molar ratio (ligand/sample). The resulting isotherm is fitted to a binding model to generate the affinity (KD), stoichiometry (n) and enthalpy of interaction (ΔH).

MicroCal PEAQ ITC Microcalorimeters for microcalorimetry and label free analysis using isothermal titration calorimetry

MicroCal PEAQ ITC Microcalorimeters for microcalorimetry and label free analysis using isothermal titration calorimetry

Specification

Measurement type:
Affinity (KD)
Measurement type:
Enthalpy ∆H
Measurement type:
Entropy ∆S
Measurement type:
Stoichiometry (n)
Sample volume:
280 µL
Cell volume:
200 µL
Injection syringe volume:
40 µL
Injection volume precision:
< 1% @ 2 µL
Sample throughput:
0-12 per 8 h day
Cell material:
Hastelloy
Cell:
Coin-shaped
Noise:
0.15 ncal/s
Temperature range:
2°C to 80°C
Temperature stability:
± 0.00012°C
Response time:
8 s*
Multiple feedback modes:
Yes (passive, high gain, low gain)
Notes:
*The MicroCal PEAQ-ITC Instrument Response Time is a true time constant.  It is the time interval between the first deviation away from the baseline, and the point on the peak that is 63% of the maximum peak height.

Operating Environment

Temperature (operating):
10°C to 28°C
Humidity:
0% to 70% RH, non condensing

Electrical ratings

Power:
100 - 200 V, 50/60 Hz, 130 W
Weight:
13.6 kg
Dimensions (W, D, H):
43 x 38 x 46 cm (calorimeter + wash station)

Support

Protect your investment and ensure optimized performance at all times with Malvern Panalytical's service plans.

When you purchase a Malvern Panalytical product we understand that this is just the first stage of a working relationship that will last for the lifetime of the instrument. Depending on your needs, Malvern Panalytical will provide the support for your business.

Help Desk

Training

Software downloads

Malvern Panalytical's service options- choose the right plan for you:

Malvern Panalytical Platinum Plan

For laboratories where maximizing instrument up-time is critical to its daily performance. Our highest level of response and the 'all inclusive' price plan takes care of those unexpected repair bills should the unfortunate occur.

Malvern Panalytical Gold Plan

Enhance your laboratory productivity by maintaining instrument efficiency. Still receive that priority response, combined with specialist technical and software support, we'll keep your instrument performance on track to deliver.

Malvern Panalytical Bronze Visit

For laboratories looking to optimize instrument performance, regular preventative maintenance is essential.

Platinum PlanGold PlanBronze Visit
Annual PM/PV Test
yes
yes
no
Telephone / Email Support
yes
yes
no
Priority Response
yes
yes
no
Emergency Breakdown visits*
yes
yes
no
Parts Included
yes
no
no
Technical & Software Support
yes
yes
yes
IQ/OQ (Pharma)***
yes
yes
yes

*including labour & travel costs***available at an additional cost

Key applications

MicroCal PEAQ-ITC instruments provide value to a wide range of applications, some of which are outlined below.

Affinity determination of biological systems

ITC is ideal for measuring very accurate affinities and stoichiometries of biological interactions. It does not require the labelling or immobilization of the interacting molecules and has no molecular mass limitations. There are many 1000s of articles in the literature that have used ITC  to study biological interactions involving proteins, nucleic acids, antibodies, carbohydrates, lipids ATP, inhibitors and drug compounds to name some of the more common examples.

Webinar: Biophysical characterization of epigenetic interactions and associated drug discovery, using Isothermal Titration Calorimetry 

Whitepaper: Use of the new MicroCal PEAQ-ITC system for measurement and characterization of a broad range of protein-LMW compound interactions

Mechanism of Action 

Where does the ligand bind the target? At the active site? At the allosteric site?  Is the interaction competitive, non-competitive or uncompetitive with respect to other ligands and cofactors? Is the binding cooperative? ITC can give you an insight into these mechanisms of binding and interaction.

Webinar: Addressing complexity of binding interactions with ITC - get the most out of your ITC data

Webinar: Revealing kinase inhibitor mechanisms: ITC leads the way

Enzyme Kinetics

Do you have a simple assay for your enzyme? Does your assay involve complex assay set up? Does modifying your substrate interfere with your SAR or mechanistic studies. ITC can be used to study any enzyme reaction and requires only nano to picogrammes of material AND is label free. 

Webinar: Studying enzyme kinetics through Isothermal Titration Calorimetry

Webinar: Use of isothermal titration calorimetry to investigate and identify novel peptide substrates for prolyl carboxypeptidase: a technology comparison 

Critical Micelle concentration

ITC has been used extensively for the determination of CMCs for a great variety of micellar complexes including surfactants, block copolymers, surface-active peptides and proteins, as well as of amino-acid-type surfactants and amino-functionalized lipids.

Application note: Analysis of demicellization data from isothermal titration calorimetry

Webinar: Characterizing the size, aggregation number and CMC of surfactant micelles using Dynamic Light Scattering