What is Nanoparticle Tracking Analysis (NTA) Technology?
What is Nanoparticle Tracking Analysis (NTA) Technology?
NTA (Nanoparticle Tracking Analysis) uses both light scattering and Brownian motion characteristics to obtain particle size distribution in a suspension.
When a laser beam passes through the sample chamber, particles in the path of this beam scatter light, which can be easily visualized with a long-distance 20x magnification microscope equipped with a video camera. This camera captures video files of particles moving under Brownian motion. The software tracks many particles individually and calculates hydrodynamic diameter using the Stokes-Einstein equation. The NanoSight range provides high-resolution measurements of particle size, concentration, and aggregates, while the fluorescence mode provides specific results for labeled particles. This range enables the unique confirmation of all these analyses with visual validation while monitoring small changes in particle quantity in real time.
This technique can simultaneously analyze multiple characteristics, saving time and sample volume while minimizing sample preparation and equipment consumables, thus reducing routine operation costs.
Application of ASTM E2834-12(2012) standard guide for measuring nanoparticle size distribution of nanomaterials in suspension by NTA includes the following examples:
- Development of drug delivery systems
- Viral vaccines
- Nanotoxicology and biomarkers
- Protein aggregation
- Extracellular vesicles (exosomes and microvesicles)
Relevant Application Notes
- Fluorescently Labeled Nanoparticles
- Nanotoxicological and Ecotoxicological Studies(Nanomaterials Regulation)
- Nanoparticles of Drug Delivery and Targeting
- Extracellular Vesicles(Exosomes and Microvesicles)
This article may have been translated automatically
{{ product.product_name }}
{{ product.product_strapline }}
{{ product.product_lede }}
