Particle concentration

Simultaneous measurement of particle concentration and size

Measurement of particle concentration is a ubiquitous requirement across a diverse range of applications. In many cases knowing just particle size is not enough, as sample concentration may also affect the performance of the product or the biological response to a drug delivery vector for example. 

In addition, there is an increasing requirement to measure nanoparticle concentration which is being driven by the EU definition of a Nanomaterial. This definition stipulates that a sample containing 50% or more of its particles by a number distribution, with a size smaller than 100nm, may need to be identified as a nanomaterial and thus regulated as such.


There are a wide variety of applications in which the measurement of particle/nanoparticle concentration  is critically important, including:

  • Biopharmaceutical (including vaccine) development, manufacture and quality assurance, in which the titer of the virus is related to the viral dosage and resultant performance of the vaccine.
  • Protein aggregation  studies, including accelerated stress testing and stability studies.
  • Differentiation and concentration measurement of sub-visible protein aggregates and silicone oil droplets in pre-filled syringe aggregation studies.
  • Drug delivery in which the biological response to the drug may be influenced by the concentration and particle size of the delivery vector.
  • Exosomes and microvesicle  research in which the concentration of specific exosomes and microvesicles may be indicative of the onset of disease and therefore is of interest in the area of disease diagnostics.  
  • Nanoparticle toxicology in which the concentration of particles within a biological or ecological environment may influence the biological response to the nanoparticles from a toxicity perspective.
  • Regulatory requirements as a result of the EU definition of a nanomaterial in which number-based distributions determine whether a material is classed as a nanomaterial or not.

Our technologies

Malvern Panalytical supplies unique technologies for measuring particle concentration: Nanoparticle Tracking Analysis (NTA) and Dynamic Light Scattering (DLS). 

These technologies provide concentration measurement across a wide dynamic range of concentrations and sizes, with a range of complimentary measurements also available such as particle size analysis and ensemble particle zeta potential measurement, particle mass measurement and particle fluorescence.

Zeta potential

Zeta potential measurements to improve formulation stability and shelf life...
Zeta potential
NanoSight range

NanoSight range

Visually track size and count nanoparticles

Zetasizer Advance Range

Zetasizer Advance Range

Light Scattering for every application

Measurement type
Particle concentration
Particle size
Protein aggregation
Molecular size
Zeta potential
Nanoparticle Tracking Analysis
Dynamic Light Scattering
Electrophoretic Light Scattering