NanoSight Pro

Overview

NanoSight Pro is powered by decades of innovation from the leaders in Nanoparticle Tracking Analysis (NTA) – no wonder it’s the most widely cited NTA solution in peer-reviewed biomaterials research. Why settle for anything else, when you can choose NanoSight Pro?

Quantify nanoparticle size and concentration to assess quality and stability in minutes
Detect fluorescently labeled subpopulations, identifying biomarkers, cargo, and nanoparticles in complex matrices
Guided workflows, flow measurement & AI-driven analysis ensure consistency across users and sites
Visualize particles to immediately validate results and trust the data behind your measurements
Ensure 21 CFR Part 11 compliance with OmniTrust

NanoSight Pro key features

Validate every result: see your particles as they’re tracked

NanoSight Pro shows you exactly what’s happening in your sample, in real time. Transparent particle tracking means you can watch each particle being detected and tracked on screen. NanoSight Pro gives you immediate confidence that the data you’re generating because it shows you exactly what’s in your sample.

As we always say, “Seeing is believing”: you can visually validate the tracking process, assess sample purity and complexity immediately, and ensure that every result is backed by visual evidence. It’s an open, trustworthy approach that makes NanoSight Pro ideal for complex or high-value samples where confidence in every data point matters.

Combine light scatter and fluorescence for deeper insights

Use light scatter mode to visualize and measure individual nanoparticles in real time - assessing size distribution, particle concentration, and aggregation state. Then switch to fluorescence mode to detect and analyze labeled subpopulations, track specific biomarkers, or quantify labeled cargo.

These complementary modes give you a more complete understanding of your sample - ideal for complex formulations, biologics, and advanced delivery systems where both total particle behavior and subpopulation insights matter.

Flow-based analysis for the most statistically robust data

NanoSight Pro’s syringe pump delivers a continuous flow of particles into the sample chamber, eliminating the need for manual repositioning or multiple measurements across zones – unlike other NTA systems.

Flow analysis also enables seamless transitions between light scatter and fluorescence modes within a single run, making it easier than ever to detect and quantify subpopulations. Combined with NanoSight Pro’s intelligent software, flow analysis ensures faster, more reproducible results with minimal effort. NanoSight Pro visualizes the tracked movement of every particle.

Smarter data analysis and deeper insight — powered by NS Xplorer

NS Xplorer software takes NTA data analysis to the next level. The Finite Track Length Adjustment (FTLA) algorithm delivers peak-to-peak resolution, even in polydisperse samples – so you can see what other NTA systems miss. Machine learning–based particle identification ensures accurate tracking and removes user subjectivity, so every result is consistent and reproducible — no manual tuning required.

Find out more about NS Xplorer

Powerful data visualization delivers insights fast

NS Xplorer also gives you powerful data visualization tools.

Trend graphs provide an instant view of size and particle count consistency across measurements, helping you spot anomalies and outliers at a glance
Decay graphs reveal fluorescent properties and photobleaching effects, offering deeper insight into particle behavior.

Combined with interactive overlays, multi-sample comparisons, and video playback, these tools make it easy to explore your data and extract meaningful results — fast.

Low Volume Flow Cell conserves precious samples

NanoSight Pro’s Low Volume Flow Cell (LVFC) enables precise flow analysis while using as little as 250 μL of sample - ideal for conserving precious or limited materials.

The LVFC is optimized for aqueous suspensions, which are commonly used in nanoparticle measurements, ensuring reliable results even with minimal sample volumes.

Its accessible, easy-to-clean design reduces downtime and minimizes the risk of cross-contamination between runs, supporting fast, reproducible workflows across a wide range of applications.

Flexible laser configuration for optimal performance and maximum uptime

NanoSight Pro features interchangeable laser modules, giving you four excitation options to match your application needs:

405 nm
488 nm
532 nm
642 nm

This flexibility supports a wide range of sample types and fluorescence labeling requirements, helping you get the most from your NTA experiments.

And because the modules are easily swapped, upgrades are simple and downtime is reduced. If a module ever needs replacement, you can get back to work faster, without sending the whole system off for repair.

Broader temperature range for stability and stress studies

NanoSight Pro offers a broad temperature range - from 10 °C below ambient up to 70 °C - giving you the flexibility to perform critical stability studies and thermal stress testing with confidence.

Whether you’re investigating temperature-sensitive nanoparticles, exploring how formulations behave under stress, or validating storage conditions, NanoSight Pro helps you generate relevant, real-world data across the temperature range that applies to your application.

Applications

Extracellular vesicles: In EV research, accurate monitoring of isolation and purification is critical. NanoSight Pro delivers rapid, high-resolution size and concentration data, while fluorescence detection helps identify EV subpopulations based on origin or composition.

Viruses & vaccines: Precise control is essential in vaccine manufacturing. NanoSight Pro enables fast, particle-by-particle sizing and counting of viruses, supporting process optimization and offering a faster alternative to titer-based assays.

Drug delivery & gene therapy: For nanoparticles like LNPs and viral vectors, NanoSight Pro provides accurate size and concentration measurements from early R&D to batch release. It supports formulation development, and stress & stability testing.

Biotherapeutics: Aggregation impacts safety and efficacy of biopharmaceuticals. NanoSight Pro detects subvisible aggregates with high resolution, helping assess product stability under stress (e.g., pH, temperature, shear), ensuring quality and safety assurance.

Nanomaterials, colloids, and nanotoxicology: As regulatory scrutiny increases, NanoSight Pro offers number-based particle concentration and detailed size distributions for those working with engineered nanomaterials in industrial, environmental, or toxicological contexts.

Ultrafine bubbles: Ultrafine bubbles (also known as nanobubbles) are used in diverse applications, including industrial cleaning & water treatment, agriculture and food, and medical applications. However, their small size and low concentration challenge conventional techniques. NanoSight Pro is uniquely suited to detect and analyze these difficult-to-measure particles.

Technology	Nanoparticle tracking analysis
Size range (diameter)	10 nm - 1000 nm*
Particle concentration	10⁶ to 10⁹ particles / mL **
Minimum sample volume	250 μL
Advanced concentration algorithm	Dynamic observation volume-based concentration algorithm

Product compliance	Product Laser Class 1 (BS EN 60825-1:2014) EMC Directive (EN IEC61326-1:2021) Low Voltage Directive (IEC 61010-1:2010 IEC 61010-1:2010/AMD 1:2016)
Camera	High sensitivity sCMOS (USB-3)
Laser wavelengths (max power)	405 nm (<65 mW), 488nm (<45 mW), 532nm (<50 mW), 642nm (<40 mW)
Temperature control range	10°C below ambient up to 70 °C
Temperature readout	Automatic
Syringe pump	Continuous sample flow with 1 mL disposable syringes
Dimensions (W, D, H)	250 x 350 x 340 mm
Weight	Instrument: 11 kg Laser module: 1.6 kg
Power	AC 110 – 240 V, 50-60 Hz, 4.0A
Ambient conditions	Up to 80% rH at 31 °C, then decreasing linearly to 50% at 40 °C

Options	Fluorescence – automatic selection*** for up to 5 filters

Notes	* Dependent on sample and instrument configuration Sample dependent * Optional functionality; long-pass filters are available for each laser wavelength

Trusted by scientists worldwide

With over 9,300 peer-reviewed citations, NanoSight is the most widely used NTA solution in biomaterials research. As the latest evolution of the NanoSight family, NanoSight Pro is powered by decades of innovation from the leaders in NTA. Why settle for anything else when you can choose NanoSight Pro?

Unparalleled in particle-by-particle analysis

Measure individual particles and report concentration by size for fast, accurate assessment of product quality and stability. Proprietary algorithms and our unique “flow analysis” method delivers more statistically robust data compared to other NTA systems.

Customer Story: Characterizing molecularly imprinted polymers

Oliver Huseyin, of MPI Discovery, explains how he uses NanoSight Pro to develop molecularly imprinted polymers (MIP).

Our biggest challenge is the size of our molecularly imprinted polymers. Wwe need to make sure that our products are the size that we say they are. We can make sure, using NanoSight Pro that each batch is the correct size.”

Oliver Huseyin — MPI Discovery

Precise subpopulation detection

Identify specific biomarkers, phenotypes, and labelled cargo – even in complex matrices – using powerful fluorescence detection. Visualize subpopulations in real time and extract data instantly.

Customer Story: Characterizing extracellular vesicles

Prof. Rebecca Dragovic from the University of Oxford explains how NanoSight Pro’s reproducible data, and fluorescence capabilities enhance her research.

In terms of accuracy, I found that I’m getting very reproducible data across the board, in terms of intra- and inter- variability.”

Professor Rebecca Dragovic — University of Oxford

Expert results from day one

Our guided workflow and machine learning-powered NS Xplorer software help eliminate user subjectivity. From setup to analysis, each step is optimized to deliver reliable, reproducible results – whether you're a new user or an experienced scientist.

It basically makes the new user as expert as those of us who developed the technique. So, you are going to generate the same quality data at the push of a button.”

Bob Carr — Inventor of NTA technology

Start your analysis with confidence

Transform your team into nanoparticle experts with the world’s most sensitive NTA system. From expert-guided setup to intelligent software tools, NanoSight Pro brings clarity and confidence to every measurement.

Our guided workflow is based on 20 years of experience in NTA, helping you remove user subjectivity and optimize every measurement.