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Protein Stable SUPR-DSF

Differential Scanning Fluorimetry for high-throughput protein stability screening

The SUPR-DSF powers high-throughput differential scanning fluorimetry by condensing sample preparation and protein stability analysis into a single microplate. Using precision optical components to deliver the highest sensitivity, intrinsic fluorescence of proteins and full spectrum detection, both thermal ramping and isothermal chemical denaturation experiments can be easily run in 384-well microplates.

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Overview

The SUPR-DSF delivers high-throughput protein stability screening in a simple way, cutting the time needed for sample preparation, reducing the amount and cost of consumables, and minimizing the risk of error.

By reading the shift in intrinsic fluorescence of protein samples as they unfold directly in-plate, the SUPR-DSF eliminates several steps seen in other techniques. Sample preparation starts and stops in a single 384-well plate and once this is loaded onto the thermal block in the drawer at the front of the instrument, you just load a method and start a run in the SUPR-Suite software.  Once your run is complete, analysis or export of the data are just a few clicks away.

Features and benefits

Protein stability screens can reveal significant differences between the melting temperatures of samples, and the SUPR-DSF can spot even smaller variations. The use of a spectrometer to detect full emission of the intrinsic tryptophan and tyrosine residues elucidates the differences in far greater detail. 

The use of full emission spectra translates into better control over experimental output, lower signal-to-noise in the resulting data, increased reliability and more information. There are several analysis methods available, including traditional ratio methods using two wavelengths, or more comprehensive methods using the spectra. Automatic analysis breezes through 384 samples, and should you wish to use external programs post-analysis, exporting data is straightforward.

Preparing your samples and then analyzing them in the same microplate minimizes the number of steps in the stability screening method, which reduces operator interaction and risk of error. But the real efficiencies are realized in the running costs. All you need to run the SUPR-DSF is a standard black 384-well PCR plate and a plate seal. There are no extra vessels to load the samples into, and no cartridges or structures to hold them in place. 

Additionally, using a PCR plate means that the required sample volumes is as little as 10 µl and with protein concentration requirements also low, savings extend to the sample you need to prepare.

Key applications

Protein engineering and construct screening

High-throughput protein stability screening of construct libraries is an established technique used to quickly identify the best candidates for downstream processing. Ensuring the most stable form of the protein is a solid basis for further optimization work before sending candidates to the next stage of development. This process is traditionally time-consuming and expensive and often involves thousands of molecules. 

With the SUPR-DSF, stability screening has never been more efficient. High-throughput differential scanning fluorimetry using 384-well microplate technology means quick screening of many constructs, without the added workload and cost associated with other techniques. Also particularly convenient at this stage is the low sample requirement, in terms of both volume and concentration. 

Formulation and stability prediction

Once candidates have passed through early development and optimization stages, it is important to map out how they should be stored and transported, and what their safe and effective shelf-life will be. Testing multiple formulations under a range of conditions is essential to the understanding and prediction of long-term stability. Automatable microplate reader technology can quickly rank all formulations in terms of stability. Fast, data-rich information provides insight for both thermodynamic and kinetic stabilities, enabling confident decisions.

Protein-ligand binding studies

Binding analysis studies are a crucial area of drug development when screening for hits or working on lead optimization. Characterization of the interaction between protein target and ligand, and the KD (dissociation constant) parameter are critical in selecting the best candidates for further development. Thanks to the high data quality provided by the SUPR-DSF, false positives or negatives are eliminated, removing concerns about progressing candidates with non-specific binding. 

Interaction analysis by the SUPR-DSF offers label-free measurements across a wide analyte binding concentration range and in a broad range of buffer conditions. The measurements are free in solution and do not require significant assay development, providing the perfect complementary data to more traditional binding analysis techniques. With high throughput, low sample requirements, and easy processing in a 384-well plate format, binding analysis has never been easier or more robust.

Specification

Sample presentation384-well microplate (PCR plate)
Sample volumeTypically 10 µL – 30 µL
Sample concentrationMinimum 0.1 mg/mL
Temperature range10˚C - 105˚C
Thermal ramp ratesVariable; maximum 1˚C/min for all 384 wells
Typical run time80 minutes/plate
Light sourceUV LED
Excitation wavelength280 nm
Emission wavelengths measured310 nm – 420 nm
Dimensions (W, D, H)420 mm x 520 mm x 350 mm
Weight35 kg

Support

Services

Support to protect the return on your investment

To ensure that your instrument remains in excellent condition and continually delivers excellent performance, we offer a full range of expertise and support services.

Support

Service for a lifetime

  • Phone and remote support
  • Preventive maintenance and checkups
  • Flexible Customer Care Agreements
  • Performance certificates
  • Hardware and software upgrades
  • Local and global support

Expertise

Adding value to your processes 

  • Sample preparation development/optimization
  • Analytical methodologies 
  • Turnkey solutions
  • Operations via IQ/OQ/PQ, quality assurance (GLP, ISO17025) or round robins/inter-laboratory studies
  • Consultancy services

Training and education

  • Training on-site or at our competence centers
  • Broad range of basic and advanced courses on products, applications and software