| 00:00:00 | How to monitor protein stability using light scattering techniques |
| 00:02:40 | How to monitor protein stability using light scattering techniques |
| 00:02:50 | Chemical and structural integrity of proteins |
| 00:03:33 | Therapeutic protein product stability |
| 00:04:02 | Protein Aggregation – Why The Concern? |
| 00:05:13 | Dynamic Light Scattering |
| 00:06:30 | What is measured with DLS – hydrodynamic size |
| 00:07:57 | Parameters that are used in the following SlideData |
| 00:08:43 | Light scattering is sensitive to size |
| 00:09:32 | Case studies |
| 00:09:54 | Stability study of Recombinant Human Serum Albumins |
| 00:10:35 | Comparison Albucult™ and Recombumin™ |
| 00:12:47 | Electrostatic/Virial effect at high protein concentration |
| 00:14:44 | Polydispersity Index |
| 00:15:30 | Correlogram of low concentration samples of Recombumin |
| 00:16:05 | Comparison of size distributions at low concentrations |
| 00:16:33 | Recombumin™ in PBS |
| 00:17:26 | Albucult™ thermal trends |
| 00:18:14 | Protein peak size during thermal trend |
| 00:19:10 | Recombumin™ thermal trends |
| 00:19:49 | Recombumin™ thermal trends in different buffers |
| 00:20:45 | Recombumin™ formulation buffer on its own |
| 00:21:22 | pH stability test recombinant human serum albumin |
| 00:22:06 | DLS results show similar trend as GP.HPLC results |
| 00:22:42 | Summary Recombumin™ & Albucult™ samples |
| 00:23:21 | Hemoglobin based oxygen carriers (HBOCs) |
| 00:24:35 | Globin calibration curve-to estimate molecular weight |
| 00:26:05 | Globin based-calibration curve: |
| 00:26:39 | PEGylated hemoglobin data |
| 00:28:18 | Modification of proteins: PEGylated hemoglobins |
| 00:29:38 | Summary HBOCs |
| 00:30:06 | Dynamic light scattering and protein stability |
| 00:30:52 | Thank you! |
| 00:31:25 | Comparison of size distributions at low concentrations |
| 00:33:06 | Comparison Albucult™ and Recombumin™ |
| 00:34:23 | Contact Information |
A presentation of how protein stability can be monitored using light scattering. Typical examples of applications such as detection of aggregates in protein formulations and the study of thermal or chemical stability of proteins will be given