Protein zeta potential measurements using Malvern’s new diffusion barrier method

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00:00:00 Protein zeta potential measurements using Malvern’s new diffusion barrier method
00:02:10 Protein zeta potential measurements using Malvern’s new diffusion barrier method
00:02:22 Outline
00:02:53 Zeta Potential (ZP)
00:04:18 Measuring Zeta Potential
00:05:09 Zeta potential measurements using micro-electrophoresis
00:06:14 Laser Doppler Electrophoresis
00:07:12 Measurement strategy (M3-FFR)
00:08:55 Henry’s Equation
00:09:35 Zeta potential measurements of proteins
00:10:10 Diffusion barrier technique
00:12:15 Diffusion barrier technique
00:13:54 HSA measurement – full cell
00:15:20 HSA measurement – diffusion barrier
00:16:00 Size distributions before and after measurements
00:16:43 Important parameters for protein zeta potential
00:17:38 Investigating MEP using BSA in PBS+1mM DTT(4) Polarisation concentration effects
00:19:08 Investigating MEP using BSA in PBS+1mM DTT(5)
00:20:42 HSA in different pH buffers
00:21:00 HSA sample measured with diffusion barrier
00:21:41 General recommendations for MEP/zeta potential of protein samples in folded capillary cell
00:23:21 Conclusions
00:24:09 Thanks for listening
00:27:13 Contact Information
The Zetasizer Nano uses a new diffusion barrier method to protect proteins during a measurement of their charge. The presentation outlines the method and presents a number of application examples