Table of contents
3. Physical Stability and Aggregation of Antibody-Drug Conjugates
00:07
5. Therapeutic proteins offer treatments for growing number of diseases
00:35
6. Protein aggregation
00:43
7. Antibody-Drug Conjugates: A New Class of Therapeutic Agents
01:01
8. Current Conjugation Strategies
01:19
9. Challenges specific to antibody-drug conjugates
01:19
11. Types of reactions for synthesizing trastuzumab-DM1 (T-DM1)
01:28
12. Difference in intact mass analyses between T-DM1 vs Syn-ADC
01:43
13. Sample preparation
00:35
14. Effects of drug and linker conjugation on higher order structure of mAb
02:09
15. Thermal Stability using Differential Scanning Calorimetry
01:07
16. Heating-rate dependence of DSC thermograms
00:30
17. Apparent Tm Versus DSC Heating Race
00:49
18. Energy of Activation (Ea) for Heating Induced Unfolding/Aggregation Reaction
00:22
19. Plot of 1 𝑇 𝑚 vs ln 𝑣 𝑇 𝑚 2 and Energies of Activation
00:35
20. Heating induced aggregation using Dynamic Light Scattering
00:46
21. Tertiary and secondary structural changes for thermally stressed TmAb and T-DM1
00:55
22. Apparent solubility of ADCs and unconjugated mAb
00:38
23. PEG precipitation assay in PBS buffer, pH 7.4
00:49
26. Chemical denaturation of TmAb and T-DM1 to assess conformational stabilities
00:29
27. Colloidal Stability of proteins
00:54
28. Measurement of colloidal stability using light scattering technique
00:48
29. Colloidal stability of TmAb and T-DM1
01:28
31. Accelerated stress conditions
00:25
32. Aggregation of TmAb and T-DM1 During Isothermal Storage
00:39
33. Size exclusion chromatography for TmAb and T-DM1 samples stored at 50°C
01:17
34. Effects of isothermal incubation on sub-visible particles in mAb and ADCFlow-imaging microscopy
00:24
35. Effects of Isothermal Incubation on Sub-Visible Particles in mAb and ADC Resonant Mass Measurements
01:07
36. Agitation induced aggregation of TmAb and T-DM1
00:43
37. Aggregate Characterization Using SEC
01:17
38. Aggregate Characterization Using SEC
00:25
39. Micro-Particle Characterization Using Flow Imaging Microscopy
00:58
40. Micro-Particle Size Distribution
00:05
41. Sub-visible Particle Characterization Using Resonant Mass Measurement
00:27
42. Summary of results
00:42
44. Acknowledgements
00:24
46. Thank you for your attentioncraig.sagar@malvernpanalytical.com
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