Latest Trends in Intermolecular Interaction Analysis ITC
Verna Frasca, the Application Manager for MicroCal products, conducted a study of papers related to ITC using Google Scholar up to June 30, 2018. Here are the findings.
Read this article in English here
What is ITC? ~ The Gold Standard for Analyzing Intermolecular Interactions ~
ITC is considered the gold standard for analyzing intermolecular interactions of biomolecules like proteins, nucleic acids, and lipids. It not only measures binding strength but also enables the determination of stoichiometry and thermodynamic parameters.
The History of ITC ~ MicroCal’s First ITC in the World ~
Thousands of ITC units are used in life science research facilities and by small molecule and biopharmaceutical manufacturers worldwide.
ITC Papers Study ~ Number of Papers Using ITC Dramatically Increased ~
Using Google Scholar, searching for “MicroCal + (list ITC product names with ‘or’)” every five years yielded the following results.
- 1988-1992: 15 hits
- 1993-1997: 246 hits
- 1998-2002: 857 hits
- 2003-2007: 2250 hits
- 2008-2012: 4360 hits
- 2013-2018: 5800 hits
You can see the exponential rise in the number of papers using MicroCal ITC.
Closer inspection of these papers reveals measurement of various types of intermolecular interactions. Examples include protein-small molecule, protein-protein, protein-DNA, antigen-antibody, and enzyme-substrate interactions. ITC is commonly used for evaluating the binding of drug candidates with their target molecules (mainly proteins). The evaluation in fields such as nanoparticles, polymers, membranes, surfactants, and micelles is also increasing.
These are published in over 350 publications, of which 32 publications contained over 25 references using the MicroCal ITC system from January 2015 to the end of June 2018. (Number of references rounded)
Examples of ITC Measurements Published in Journals
Below are examples of ITC measurements published in various journals. (All in English)
Acta parasitological: “Measurement of binding strength between prey proteins interacting with Toxoplasma gondii SAG1 and SAG2 using isothermal titration calorimetry (ITC)“
AIDS: “Design of a highly potent HIV-1 fusion inhibitor targeting the gp41 pocket“
Allergy: “Characterization of maize chitinase‐A, a tough allergenic molecule“
Anesthesiology: “Discovery of a novel general anesthetic chemotype using high-throughput screening”
Archives of Toxicology: “Interaction of perfluoroalkyl acids with human liver fatty acid-binding protein”
Bioengineering & Translational Medicine: “Targeting HPV‐infected cervical cancer cells with PEGylated liposomes encapsulating siRNA and the role of siRNA complexation with polyethylenimine“
Biomedicine & Pharmacotherapy: “Nanobodies targeting cortactin proline rich, helical and actin binding regions downregulate invadopodium formation and matrix degradation in SCC-61 cancer cells”
Blood: “Structural basis for quinine-dependent antibody binding to platelet integrin αIIbβ3”
Breast Cancer Research: “Gossypol has anti-cancer effects by dual-targeting MDM2 and VEGF in human breast cancer”
Cell Host & Microbe: “Structural conservation despite huge sequence diversity allows EPCR binding by the PfEMP1 family implicated in severe childhood malaria”
Cerebral Cortex: “Cortical layer inversion and deregulation of reelin signaling in the absence of SOCS6 and SOCS7”
Chemical Research in Toxicology: “Identification and Molecular Interaction Studies of Thyroid Hormone Receptor Disruptors among Household Dust Contaminants”
Chemistry Select: “An ITC Study on the Interaction Energy Between Galactomannan Biopolymers and Selected MO2 Nanoparticles in Hydrogels”
ChemNanoMat: “The Effect of PEGylation on Multivalent Binding: A Surface Plasmon Resonance and Isothermal Titration Calorimetry Study with Structurally Diverse PEG‐Dendritic GATG Copolymers”
Chirality: “Isothermal Titration Calorimetry of Chiral Polymeric Nanoparticles”
Clinical and Translational Oncology: “Selection of DNA aptamers for extra cellular domain of human epidermal growth factor receptor 2 to detect HER2 positive carcinomas”
Cogent Biology: “Isolation and purification of phytocystatin from almond: Biochemical, biophysical, and immunological characterization”
Colloid and Polymer Science: “Adsorption of lysozyme on pH-responsive PnBA-b-PAA polymeric nanoparticles: studies by stopped-flow SAXS and ITC”
Extremophiles: “High-affinity RNA binding by a hyperthermophilic single-stranded DNA-binding protein”
Flavour and Fragrance Journal: “Facile separation of cinnamyl acetate and cinnamaldehyde based on host–guest complexation with β‐cyclodextrin”
Food Chemistry: “Effect of the addition of mannoproteins on the interaction between wine flavonols and salivary proteins”
Food Research International: “Evaluation of butyrylcholinesterase inhibitory activity by chlorogenic acids and coffee extracts assessed in ITC and docking simulation models”
Journal of Agricultural and Food Chemistry: “Ethanol Concentration Influences the Mechanisms of Wine Tannin Interactions with Poly(l-proline) in Model Wine”
Water, Air, & Soil Pollution: “Exploring the Effect of Cu2+ on Sludge Hydrolysis and Interaction Mechanism between Cu2+ and Xylanase by Multispectral and Thermodynamic Methods”
Below are publications issued after 2015 that include chapters on protocols using MicroCal ITC.
AMPK (Methods in Molecular Biology, volume 1732): “Applications of NMR and ITC for the Study of the Kinetics of Carbohydrate Binding by AMPK β-Subunit Carbohydrate-Binding Modules”
Antimicrobial Peptides (Methods in Molecular Biology, volume 1548): “Calorimetry Methods to Study Membrane Interactions and Perturbations Induced by Antimicrobial Host Defense Peptides”
Applied Biophysics for Drug Discovery: “Thermodynamics in Drug Discovery”
Biocalorimetry (Methods, volume 76):
Biocalorimetry: Foundations and Contemporary Approaches
Biochemical Roles of Eukaryotic Cell Surface Macromolecules (Advances in Experimental Medicine and Biology, volume 842): “Biophysical Characterization of the Interaction of O-acylcholines with the Major Bovine Seminal Plasma Protein, PDC-109”
c-Di-GMP Signaling (Methods in Molecular Biology, volume 1657): “Isothermal Titration Calorimetry to Determine Apparent Dissociation Constants (Kd) and Stoichiometry of Interaction (n) of C-di-GMP Binding Proteins”
Calorimetry (Methods in Enzymology, volume 567)
Current Protocols in Nucleic Acid Chemistry: “Calorimetry of Nucleic Acids”
Excipient Applications in Formulation Design and Drug Delivery: “Drug Excipient interactions”
Glycosaminoglycans: “Using Isothermal Titration Calorimetry to Determine Thermodynamic Parameters of Protein–Glycosaminoglycan Interaction”
In Vitro Mutagenesis (Methods in Molecular Biology, volume 1498): “Biochemical and Biophysical Methods to Examine the Effects of Site-Directed Mutagenesis on Enzymatic Activities and Interprotein Interactions”
Molecular Physiology and Ecophysiology of Sulfur: “Investigation of Protein-Protein Interactions of Ferredoxin and Sulfite Reductase Under Different Sodium Chloride Concentrations by NMR Spectroscopy and Isothermal Titration Calorimetry”
Nucleic Acid Aptamers (Methods in Molecular Biology, volume 1380): “Label-free determination of the dissociation constant of small molecule-aptamer interaction by isothermal titration calorimetry”
Nucleic Acid Crystallography (Methods in Molecular Biology, volume 1320): “Isothermal Titration Calorimetry: Assisted Crystallization of RNA–Ligand Complexes”
Polymer Micelles: “Micellization Thermodynamics of Pluronic P123 (EO20PO70EO20) Amphiphilic Block Copolymer in Aqueous Ethylammonium Nitrate (EAN) Solutions”
Potassium Channels (Methods in Molecular Biology, volume 1684): “Ion Binding to Transport Proteins using Isothermal Titration Calorimetry”
Semaphorin Signaling Methods (Methods in Molecular Biology, volume 1493): “Characterizing Plexin GTPase Interactions Using Gel Filtration, Surface Plasmon Resonance Spectrometry, and Isothermal Titration Calorimetry”
SH2 Domains (Methods in Molecular Biology, volume 1555): “Calorimetric measurement of SH2 domain ligand affinities”
Thermodynamics and Kinetics of Drug Binding: “Thermodynamic Profiling of Carbonic Anhydrase Inhibitors”
Vitamin C: “Encapsulation of Vitamin C into β‐Cyclodextrin for Advanced and Regulatory Release”
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