Overcoming Challenges in LNP Vector Analysis: Key Tools, Techniques, and Insights

I want to evaluate LNP, but I’m not sure what kind of evaluation can be done…

There seems to be some difference between the batches, but I’m not sure what it is…

If you have such concerns, please be sure to check out this eBook on LNP vector analysis.

From the perspective of property evaluation, you can also check the technological background and how DLS, ELS, SEC, and DSC work!

Table of Contents

• Chapter 1: Evaluation of LNP Size, Polydispersity, and Concentration
  • Size
  • Polydispersity
  • Concentration
  • Section Overview
• Chapter 2: Evaluation of Vector Surface Characteristics
  • Measurement of Surface Charge: Electrophoretic Light Scattering (ELS)
  • Section Overview
• Chapter 3: Understanding LNP Vector Composition (Quantification of Therapeutic Payload)
  • SEC with Inline Detector: A Key Tool for Quantifying LNP Payload
  • Section Summary
• Chapter 4: Examining the Thermal Stability of Vectors
  • Measurement of Thermal Stability by Differential Scanning Calorimeter (DSC)
  • Key Points When Using DSC
  • Section Overview
• Optimal Evaluation of LNP Vectors with Appropriate Analytical Tools

Introduction

Lipid nanoparticles (LNPs) hold great potential for treating, curing, and preventing various difficult diseases, from genetic disorders to cancer.

These vectors enable efficient delivery of therapeutic drugs like RNA, and unlike viral vector production, they can be manufactured using a cell-free production process, allowing for rapid scaling up.

However, LNP therapies pose challenges in analysis during development and manufacturing due to the complexity of their structure. To evaluate the stability of LNPs, it is necessary to accurately measure and carefully control various critical properties.

Additionally, product design, production process optimization, and release specifications must be directed and informed.

For more information, please see the eBook.