OMNISEC

Features and benefits

OMNISEC is a powerful tool for taking control of the performance, quality and value of your product, whether that’s a synthetic polymer in industry, a natural polymer or polysaccharide used in food or pharmaceutical products, or a protein or antibody used in biopharmaceuticals.

As a research tool, it accelerates research into these molecules by providing absolute information for a wide range of applications making it an ideal tool for universities. It reduces service expense and downtime and increases productivity with easy-to-use intuitive software.

Market leading high sensitive light scattering

• Measure low molecular weight samples with greater accuracy (down to 200 g/mol)
• Measure lower sample concentrations (newly synthesized polymers and proteins)
• Measure low dn/dc samples with greater accuracy and sensitivity 

High sensitivity Refractive index 

• Greater accuracy and less subjectivity at lower concentrations
• Measure lower concentraions of highly viscous materials to prevent column overload

Integrated robust differential viscometer 

• Measure Iv of samples to determine structure and branching 
• Robust to all GPC solvents and a wide pH range broadening available applications 

Mechanical viscometer bridge autobalance

• Improved viscometer data quality and accuracy

Fully integrated detector module 

• Better temperature control and reduced interdetecor volume = better chromatography 
• Improved separation quality and resolution

All detectors in series 

• no detectors in a parallel flow path meaning higher sensitivity and better quality results

Work flow orientated software

• Makes GPC/SEC measurements and analysis easy and intuitive

How it works

In size-exclusion chromatography, molecules are separated according to their hydrodynamic radius as they enter and exit the pores of a porous gel packing matrix in the column. Like other HPLC-based techniques, this involves the use of a pump, column and at least one detector to measure the sample as it elutes. An OMNISEC GPC/SEC system includes the following components:

Degasser

• Rapid mobile-phase exchange thanks to low volume degasser

Pump

• Rapid mobile-phase exchange thanks to self-priming pump
• Reduced service requirement thanks to novel continuous back seal flushing mechanism

Autosampler

• Protect samples from aggregation with autosampler temperature control
• Use your preferred sample format; vials or 96-well microtiter plates
• Reduce sample use with zero-waste mode

Column Oven

• Temperature control of up to 6 analytical columns

Refractive Index

• Measure the concentration of any solute with excellent sensitivity

UV/Vis PDA

• Measure absorbance at any wavelength from 190-900 nm
• Assess changes in absorbance spectra
• Characterize copolymers

Light Scattering

• Measure absolute molecular weight in a wider range of applications
• Measure as little as 100 ng of material
• Measure molecular weights down to 200 Da

Viscometer

• Better intrinsic viscosity measurements thanks to automated balancing
• Reduced service cost and time thanks to user-exchangeable capillaries

Specification

OMNISEC RESOLVE

Pump flow rate:

0.005 - 10 mL/min

Flow rate accuracy:

±1%

Pressure range:

0 - 5000 PSI (34.5 MPa)

Pulsation:

0.15% @ 1 mL/min in water

Dimensions (W, D, H):

420, 640, 890 mm

Weight:

62 kg

Software:

OMNISEC software v10 (or later)

Data acquisition rate:

100 Hz

AUTOSAMPLER

Capacity:

Up to 192

Sample container types:

HPLC vials or 96-well microtiter plates

Injection volume:

1 - 300 µL

Injection volume accuracy:

>99.5%

Injection volume precision:

<0.3% RSD in full loop mode

<0.5% RSD in partial loop mode

<1% RSD in µL pickup mode

Injection overhead volume:

0 µL in µL pickup mode

Syringe volume:

250 µL standard

Temperature control range:

4 - 60 °C

COLUMN OVEN

Column capacity:

6 x analytical (1 x Tricorn 10/300 GL)

Temperature control range:

20 - 65 °C

OMNISEC REVEAL

Dimensions (W, D, H):

420, 640, 600 mm

Weight:

40 kg

Detector temperature range:

20 - 65 °C

Software:

OMNISEC software v10 (or later)

Data acquisition rate:

100 Hz

Patents:

US20140060162A1 & EP2619543Ba "Modular capillary bridge viscometer"

US20140144214A1 & EP2619544A1 "Automatically balanced capillary bridge viscometer"

Detector 1

Detector:

Light scattering

Measurement principle:

RALS 90° angle, LALS 7° angle

Light source:

50 mW, 640 nm laser

Cell volume:

18 µL

Dynamic range:

2500 mV

Baseline noise:

<0.1 mV

Baseline drift:

<0.2 mV/hr

Detector 2

Detector:

Differential refractive index

Measurement principle:

Deflection

Cell volume:

12 µL

Dynamic range:

±2.5 x 10^-4 RIU

Baseline noise:

<10^-7 RIU

Baseline drift:

<3 x 10^-7 RIU/hr

Detector 3

Detector:

Viscometer

Measurement principle:

4-capillary Wheatstone bridge with self-balancing mechanism and user-exchangeable capillaries

Transducers:

Firmware-based transducer overpressure protection

Detector volume:

17 µL/capillary

Differential pressure baseline noise:

0.3 Pa

Inlet pressure dynamic range:

100 kPa

Inlet pressure baseline noise:

0.01 kPa

Baseline drift:

<0.2 kPa/hr

Detector 4

Detector:

Diode-array-based UV/Vis spectrometer

Wavelength:

190 - 900 nm

Number of wavelengths:

1024

Wavelength accuracy:

<1 nm

Wavelength resolution:

0.6 nm

Cell volume:

7.5 µL

Path length:

10 mm

Baseline noise:

2 x 10^-5 AU

Baseline drift:

5 x 10^-4 AU/hr

Measurement type 1

Measurement type:

Absolute molecular weight

Measurement range:

200 - >10⁷ g/mol

Minimum quantifiable mass:

100 ng of 100kDa molecular weight polystyrene in THF

Measurement principle:

Light scattering (LALS or RALS)

Measurement type 2

Measurement type:

Intrinsic viscosity

Minimum quantifiable mass:

1 µg of 100kDa molecular weight polystyrene in THF

Measurement principle:

4-capillary Wheatstone bridge

Measurement type 3

Measurement type:

Concentration

Minimum quantifiable mass:

100 ng of 100kDa molecular weight polystyrene in THF

Measurement principle:

Differential refractive index detection

Operating environment

Temperature:

15 - 30 °C

Software

Minimum computer specification:

Windows 7 Professional 64 bit, 4th Gen Intel(R) Core I7 Processor (Quad Core HT, 3.4 GHz Turbo, w/ HD Graphics), 8 GB 1600 MHz DDR3 memory, 500 GB 3.5 inch SATA (7200 RPM) HDD, Full-HD monitor

Accessories

OMNISEC

OMNISEC RESOLVE

Combined pump, degasser, autosampler and column oven, for high quality GPC/SEC and accurate molecular weight measurement.

OMNISEC RESOLVE combines with OMNISEC REVEAL to form a complete OMNISEC GPC/SEC solution for the measurement of absolute molecular weight, intrinsic viscosity and other parameters. It combines an integrated pump, degasser, autosampler and column oven in a single advanced separations unit.

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OMNISEC REVEAL

The ultimate in integrated advanced detection for GPC/SEC and UPLC/UHPLC

Molecular weight, intrinsic viscosity and numerous other parameters can be measured with OMNISEC REVEAL, the world’s most advanced integrated GPC/SEC multi-detector available. It can be added to any existing conventional GPC/SEC system or used in conjunction with OMNISEC RESOLVE to form a complete GPC/SEC solution, to offer comprehensive molecular characterization of synthetic and natural polymers, as well as proteins. The system is now also compatible with UPLC/UHPLC systems such as Waters ACQUITY APC™ for faster and more detailed polymer analysis.

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SEC-MALS 20

Multi angle light scattering detector

The Viscotek SEC-MALS 20 is a multi angle light scattering detector that can be used to measure the absolute molecular weight of proteins, synthetic and natural polymers, as well as molecular size expressed as the radius of gyration, Rg.

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OMNISEC software

Powerful, agile, easy to use GPC/SEC software for OMNISEC instrument control, data acquisition, analysis and reporting

OMNISEC software is an integrated solution for the advanced analysis of proteins and polymers by GPC/SEC, and is specifically designed for control of OMNISEC RESOLVE and OMNISEC REVEAL. Simplified workflows improve productivity and help you to maximize the information and efficiency of your experiments. Simply load your samples and the software controls all the sequencing, enabling truly automatic unattended operation.

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GPC and SEC Columns

GPC/SEC columns for all aqueous and organic applications

A wide range of columns for analytical GPC/SEC of synthetic and natural polymers and proteins.

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GPC and SEC Standards

A range of molecular weight standards for different applications of GPC/SEC

Malvern Panalytical offers a wide range of molecular weight standards for GPC/SEC for conventional and universal calibration as well as light scattering.

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Support

Protect your investment and ensure optimized performance at all times with Malvern Panalytical's service plans.

When you purchase a Malvern Panalytical product we understand that this is just the first stage of a working relationship that will last for the lifetime of the instrument. Depending on your needs, Malvern Panalytical will provide the support for your business.

Help Desk

• Telephone and email support.
• Operators who take responsibility for your call and ensure your query is answered.

Training

• User training events

Software downloads

• A simple mechanism for receiving updates and new features

Malvern Panalytical's service options- choose the right plan for you:

*including labour & travel costs***available at an additional cost

Key applications

OMNISEC can offer key information to a broad range of applications including: 

Bioscience and Biopharmaceuticals

Quantify key metrics in stability profiling and biosimilarity. Accurately measure monomer, fragments and higher order aggregates independent of column retention volume for protein, viruses, VLP’s and more. Predict and understand immunogenicity and efficacy. 

Application Note: Characterization of IgG monomers and their aggregates

Polymers, Plastics and Elastomers

Control strength, toughness, durability, brittleness, processability and more to optimize the product quality for the desired application. Control product quality by understanding the effects of changing raw material supply, synthesis or manufacturing processes. 

Understand the impact of processing and reprocessing on key material properties using conventional and advanced technologies, such as additive manufacturing. 

Gain a deeper insight into degradation products, additives or impurities present due to polymer recycling processes.

Application Note: GPC/SEC Analysis of Polymer Degradation

Gene therapy 

Directly calculate critical to quality attributes for AAV samples. Percentage of full AAV, cp/vg, and viral titer are all easily accessible to enhance the process development and purification of new drug products. 

Application Note: The importance of Multi-Detection SEC in gene therapy

Bioconjugation 

Protein conjugation analysis allows accurate quantification of protein-polymer ratio, glycoprotein, and drug antibody ratios (DAR). Optimize the bioconjugation process to extend half-life, and cell permeability.  Measure protein detergent ratios of isolated membrane proteins to improve the crystallization process.

Application Note: PEGylated proteins: Optimizing conjugation reactions using triple detection SEC

Paints and Inks

The effectiveness of printed polymers is linked to their macromolecular properties such as molecular weight and structural distributions. Understand the link between printing and the degradation of polymers is critical to ensure the efficacy of the printed pattern is not negatively impacted. 

Webinar: Focus on Polymers - Characterization of Inks with GPC

Pharmaceutical 

Polymeric excipient properties relate directly to clinical efficacy, safety, and quality of the product.  Control the viscosity and molecular weight to ensure optimal performance or confirm the quality of sourced materials before they enter your production process. 

Application Note: Understanding the effect of molecular weight and structure on the performance characteristics of cellulose derivatives using OMNISEC

Drug Delivery 

Measure degradation and drug delivery rates. Drug release rates from medicines are directly linked to the structure of the polymeric material used to deliver them. Controlling these structures ensures the final payload is delivered to the correct location. 

Application Note: Optimizing the macromolecular properties of natural polymers for 3D-bioprinting applications

Food and Drinks 

Control the mouthfeel and performance of food products by manipulating the molecular properties (Mw, IV) of the bulk material. 

Optimize the depolymerization of native polysaccharides to produce specific thickening properties.

Monitoring degradation of polysaccharides due to processing or blending of different source materials. 

Application Note: Monitoring degradation of sodium alginate using GPC/SEC