OMNISEC
The world’s most advanced multi-detector GPC/SEC system
Malvern Panalytical's OMNISEC is a Gel Permeation Chromatography (GPC) / Size Exclusion Chromatography (SEC) system for the measurement of absolute molecular weight, molecular size, intrinsic viscosity, branching and other parameters. It includes OMNISEC RESOLVE, the integrated GPC/SEC module, OMNISEC REVEAL, the integrated multi-detector module and OMNISEC software for the characterization of synthetic and natural polymers, and proteins.
Features and benefits
OMNISEC is a powerful tool for taking control of the performance, quality and value of your product, whether that’s a synthetic polymer in industry, a natural polymer or polysaccharide used in food or pharmaceutical products, or a protein or antibody used in biopharmaceuticals.
As a research tool, it accelerates research into these molecules by providing absolute information for a wide range of applications making it an ideal tool for universities. It reduces service expense and downtime and increases productivity with easy-to-use intuitive software.
Market leading high sensitive light scattering
- Measure low molecular weight samples with greater accuracy (down to 200 g/mol)
- Measure lower sample concentrations (newly synthesized polymers and proteins)
- Measure low dn/dc samples with greater accuracy and sensitivity
High sensitivity Refractive index
- Greater accuracy and less subjectivity at lower concentrations
- Measure lower concentraions of highly viscous materials to prevent column overload
Integrated robust differential viscometer
- Measure Iv of samples to determine structure and branching
- Robust to all GPC solvents and a wide pH range broadening available applications
Mechanical viscometer bridge autobalance
- Improved viscometer data quality and accuracy
Fully integrated detector module
- Better temperature control and reduced interdetecor volume = better chromatography
- Improved separation quality and resolution
All detectors in series
- no detectors in a parallel flow path meaning higher sensitivity and better quality results
Work flow orientated software
- Makes GPC/SEC measurements and analysis easy and intuitive
How it works
In size-exclusion chromatography, molecules are separated according to their hydrodynamic radius as they enter and exit the pores of a porous gel packing matrix in the column. Like other HPLC-based techniques, this involves the use of a pump, column and at least one detector to measure the sample as it elutes. An OMNISEC GPC/SEC system includes the following components:
Degasser
- Rapid mobile-phase exchange thanks to low volume degasser
Pump
- Rapid mobile-phase exchange thanks to self-priming pump
- Reduced service requirement thanks to novel continuous back seal flushing mechanism
Autosampler
- Protect samples from aggregation with autosampler temperature control
- Use your preferred sample format; vials or 96-well microtiter plates
- Reduce sample use with zero-waste mode
Column Oven
- Temperature control of up to 6 analytical columns
Refractive Index
- Measure the concentration of any solute with excellent sensitivity
UV/Vis PDA
- Measure absorbance at any wavelength from 190-900 nm
- Assess changes in absorbance spectra
- Characterize copolymers
Light Scattering
- Measure absolute molecular weight in a wider range of applications
- Measure as little as 100 ng of material
- Measure molecular weights down to 200 Da
Viscometer
- Better intrinsic viscosity measurements thanks to automated balancing
- Reduced service cost and time thanks to user-exchangeable capillaries
Specification
OMNISEC RESOLVE
- Pump flow rate:
- 0.005 - 10 mL/min
- Flow rate accuracy:
- ±1%
- Pressure range:
- 0 - 5000 PSI (34.5 MPa)
- Pulsation:
- 0.15% @ 1 mL/min in water
- Dimensions (W, D, H):
- 420, 640, 890 mm
- Weight:
- 62 kg
- Software:
- OMNISEC software v10 (or later)
- Data acquisition rate:
- 100 Hz
AUTOSAMPLER
- Capacity:
- Up to 192
- Sample container types:
- HPLC vials or 96-well microtiter plates
- Injection volume:
- 1 - 300 µL
- Injection volume accuracy:
- >99.5%
- Injection volume precision:
<0.3% RSD in full loop mode
<0.5% RSD in partial loop mode
<1% RSD in µL pickup mode
- Injection overhead volume:
- 0 µL in µL pickup mode
- Syringe volume:
- 250 µL standard
- Temperature control range:
- 4 - 60 °C
COLUMN OVEN
- Column capacity:
- 6 x analytical (1 x Tricorn 10/300 GL)
- Temperature control range:
- 20 - 65 °C
OMNISEC REVEAL
- Dimensions (W, D, H):
- 420, 640, 600 mm
- Weight:
- 40 kg
- Detector temperature range:
- 20 - 65 °C
- Software:
- OMNISEC software v10 (or later)
- Data acquisition rate:
- 100 Hz
- Patents:
US20140060162A1 & EP2619543Ba "Modular capillary bridge viscometer"
US20140144214A1 & EP2619544A1 "Automatically balanced capillary bridge viscometer"
Detector 1
- Detector:
- Light scattering
- Measurement principle:
- RALS 90° angle, LALS 7° angle
- Light source:
- 50 mW, 640 nm laser
- Cell volume:
- 18 µL
- Dynamic range:
- 2500 mV
- Baseline noise:
- <0.1 mV
- Baseline drift:
- <0.2 mV/hr
Detector 2
- Detector:
- Differential refractive index
- Measurement principle:
- Deflection
- Cell volume:
- 12 µL
- Dynamic range:
- ±2.5 x 10-4 RIU
- Baseline noise:
- <10-7 RIU
- Baseline drift:
- <3 x 10-7 RIU/hr
Detector 3
- Detector:
- Viscometer
- Measurement principle:
- 4-capillary Wheatstone bridge with self-balancing mechanism and user-exchangeable capillaries
- Transducers:
- Firmware-based transducer overpressure protection
- Detector volume:
- 17 µL/capillary
- Differential pressure baseline noise:
- 0.3 Pa
- Inlet pressure dynamic range:
- 100 kPa
- Inlet pressure baseline noise:
- 0.01 kPa
- Baseline drift:
- <0.2 kPa/hr
Detector 4
- Detector:
- Diode-array-based UV/Vis spectrometer
- Wavelength:
- 190 - 900 nm
- Number of wavelengths:
- 1024
- Wavelength accuracy:
- <1 nm
- Wavelength resolution:
- 0.6 nm
- Cell volume:
- 7.5 µL
- Path length:
- 10 mm
- Baseline noise:
- 2 x 10-5 AU
- Baseline drift:
- 5 x 10-4 AU/hr
Measurement type 1
- Measurement type:
- Absolute molecular weight
- Measurement range:
- 200 - >107 g/mol
- Minimum quantifiable mass:
- 100 ng of 100kDa molecular weight polystyrene in THF
- Measurement principle:
- Light scattering (LALS or RALS)
Measurement type 2
- Measurement type:
- Intrinsic viscosity
- Minimum quantifiable mass:
- 1 µg of 100kDa molecular weight polystyrene in THF
- Measurement principle:
- 4-capillary Wheatstone bridge
Measurement type 3
- Measurement type:
- Concentration
- Minimum quantifiable mass:
- 100 ng of 100kDa molecular weight polystyrene in THF
- Measurement principle:
- Differential refractive index detection
Operating environment
- Temperature:
- 15 - 30 °C
Software
- Minimum computer specification:
- Windows 7 Professional 64 bit, 4th Gen Intel(R) Core I7 Processor (Quad Core HT, 3.4 GHz Turbo, w/ HD Graphics), 8 GB 1600 MHz DDR3 memory, 500 GB 3.5 inch SATA (7200 RPM) HDD, Full-HD monitor
Accessories
OMNISEC
OMNISEC RESOLVE
Combined pump, degasser, autosampler and column oven, for high quality GPC/SEC and accurate molecular weight measurement.
OMNISEC RESOLVE combines with OMNISEC REVEAL to form a complete OMNISEC GPC/SEC solution for the measurement of absolute molecular weight, intrinsic viscosity and other parameters. It combines an integrated pump, degasser, autosampler and column oven in a single advanced separations unit.
OMNISEC REVEAL
The ultimate in integrated advanced detection for GPC/SEC and UPLC/UHPLC
Molecular weight, intrinsic viscosity and numerous other parameters can be measured with OMNISEC REVEAL, the world’s most advanced integrated GPC/SEC multi-detector available. It can be added to any existing conventional GPC/SEC system or used in conjunction with OMNISEC RESOLVE to form a complete GPC/SEC solution, to offer comprehensive molecular characterization of synthetic and natural polymers, as well as proteins. The system is now also compatible with UPLC/UHPLC systems such as Waters ACQUITY APC™ for faster and more detailed polymer analysis.
SEC-MALS 20
Multi angle light scattering detector
The Viscotek SEC-MALS 20 is a multi angle light scattering detector that can be used to measure the absolute molecular weight of proteins, synthetic and natural polymers, as well as molecular size expressed as the radius of gyration, Rg.
OMNISEC software
Powerful, agile, easy to use GPC/SEC software for OMNISEC instrument control, data acquisition, analysis and reporting
OMNISEC software is an integrated solution for the advanced analysis of proteins and polymers by GPC/SEC, and is specifically designed for control of OMNISEC RESOLVE and OMNISEC REVEAL. Simplified workflows improve productivity and help you to maximize the information and efficiency of your experiments. Simply load your samples and the software controls all the sequencing, enabling truly automatic unattended operation.
GPC and SEC Columns
GPC/SEC columns for all aqueous and organic applications
A wide range of columns for analytical GPC/SEC of synthetic and natural polymers and proteins.
GPC and SEC Standards
A range of molecular weight standards for different applications of GPC/SEC
Malvern Panalytical offers a wide range of molecular weight standards for GPC/SEC for conventional and universal calibration as well as light scattering.
Support
Protect your investment and ensure optimized performance at all times with Malvern Panalytical's service plans.
When you purchase a Malvern Panalytical product we understand that this is just the first stage of a working relationship that will last for the lifetime of the instrument. Depending on your needs, Malvern Panalytical will provide the support for your business.
Help Desk
- Telephone and email support.
- Operators who take responsibility for your call and ensure your query is answered.
Training
Software downloads
Malvern Panalytical's service options- choose the right plan for you:
Malvern Panalytical Platinum Plan
For laboratories where maximizing instrument up-time is critical to its daily performance. Our highest level of response and the 'all inclusive' price plan takes care of those unexpected repair bills should the unfortunate occur.
Malvern Panalytical Gold Plan
Enhance your laboratory productivity by maintaining instrument efficiency. Still receive that priority response, combined with specialist technical and software support, we'll keep your instrument performance on track to deliver.
Malvern Panalytical Bronze Visit
For laboratories looking to optimize instrument performance, regular preventative maintenance is essential.
Platinum Plan | Gold Plan | Bronze Visit | |
---|---|---|---|
Annual PM/PV Test | yes | yes | no |
Telephone / Email Support | yes | yes | no |
Priority Response | yes | yes | no |
Emergency Breakdown visits* | yes | yes | no |
Parts Included | yes | no | no |
Technical & Software Support | yes | yes | yes |
IQ/OQ (Pharma)*** | yes | yes | yes |
*including labour & travel costs***available at an additional cost
Key applications
OMNISEC can offer key information to a broad range of applications including:
Bioscience and Biopharmaceuticals
Quantify key metrics in stability profiling and biosimilarity. Accurately measure monomer, fragments and higher order aggregates independent of column retention volume for protein, viruses, VLP’s and more. Predict and understand immunogenicity and efficacy.
Application Note: Characterization of IgG monomers and their aggregates
Polymers, Plastics and Elastomers
Control strength, toughness, durability, brittleness, processability and more to optimize the product quality for the desired application. Control product quality by understanding the effects of changing raw material supply, synthesis or manufacturing processes.
Understand the impact of processing and reprocessing on key material properties using conventional and advanced technologies, such as additive manufacturing.
Gain a deeper insight into degradation products, additives or impurities present due to polymer recycling processes.
Application Note: GPC/SEC Analysis of Polymer Degradation
Gene therapy
Directly calculate critical to quality attributes for AAV samples. Percentage of full AAV, cp/vg, and viral titer are all easily accessible to enhance the process development and purification of new drug products.
Application Note: The importance of Multi-Detection SEC in gene therapy
Bioconjugation
Protein conjugation analysis allows accurate quantification of protein-polymer ratio, glycoprotein, and drug antibody ratios (DAR). Optimize the bioconjugation process to extend half-life, and cell permeability. Measure protein detergent ratios of isolated membrane proteins to improve the crystallization process.
Application Note: PEGylated proteins: Optimizing conjugation reactions using triple detection SEC
Paints and Inks
The effectiveness of printed polymers is linked to their macromolecular properties such as molecular weight and structural distributions. Understand the link between printing and the degradation of polymers is critical to ensure the efficacy of the printed pattern is not negatively impacted.
Webinar: Focus on Polymers - Characterization of Inks with GPC
Pharmaceutical
Polymeric excipient properties relate directly to clinical efficacy, safety, and quality of the product. Control the viscosity and molecular weight to ensure optimal performance or confirm the quality of sourced materials before they enter your production process.
Drug Delivery
Measure degradation and drug delivery rates. Drug release rates from medicines are directly linked to the structure of the polymeric material used to deliver them. Controlling these structures ensures the final payload is delivered to the correct location.
Food and Drinks
Control the mouthfeel and performance of food products by manipulating the molecular properties (Mw, IV) of the bulk material.
Optimize the depolymerization of native polysaccharides to produce specific thickening properties.
Monitoring degradation of polysaccharides due to processing or blending of different source materials.
Application Note: Monitoring degradation of sodium alginate using GPC/SEC