OMNISEC

Features and benefits

OMNISEC is a powerful tool for taking control of the performance, quality and value of your product, whether that’s a synthetic polymer in industry, a natural polymer or polysaccharide used in food or pharmaceutical products, or a protein or antibody used in biopharmaceuticals.

As a research tool, it accelerates research into these molecules by providing absolute information for a wide range of applications making it an ideal tool for universities. It reduces service expense and downtime and increases productivity with easy-to-use intuitive software.

Market leading high sensitive light scattering

• Measure low molecular weight samples with greater accuracy (down to 200 g/mol)
• Measure lower sample concentrations (newly synthesized polymers and proteins)
• Measure low dn/dc samples with greater accuracy and sensitivity 

High sensitivity Refractive index 

• Greater accuracy and less subjectivity at lower concentrations
• Measure lower concentraions of highly viscous materials to prevent column overload

Integrated robust differential viscometer 

• Measure Iv of samples to determine structure and branching 
• Robust to all GPC solvents and a wide pH range broadening available applications 

Mechanical viscometer bridge autobalance

• Improved viscometer data quality and accuracy

Fully integrated detector module 

• Better temperature control and reduced interdetecor volume = better chromatography 
• Improved separation quality and resolution

All detectors in series 

• no detectors in a parallel flow path meaning higher sensitivity and better quality results

Work flow orientated software

• Makes GPC/SEC measurements and analysis easy and intuitive

How it works

In size-exclusion chromatography, molecules are separated according to their hydrodynamic radius as they enter and exit the pores of a porous gel packing matrix in the column. Like other HPLC-based techniques, this involves the use of a pump, column and at least one detector to measure the sample as it elutes. An OMNISEC GPC/SEC system includes the following components:

Specification

OMNISEC RESOLVE

Pump flow rate:

0.005 - 10 mL/min

Flow rate accuracy:

±1%

Pressure range:

0 - 5000 PSI (34.5 MPa)

Pulsation:

0.15% @ 1 mL/min in water

Dimensions (W, D, H):

420, 640, 890 mm

Weight:

62 kg

Software:

OMNISEC software v10 (or later)

Data acquisition rate:

100 Hz

AUTOSAMPLER

Capacity:

Up to 192

Sample container types:

HPLC vials or 96-well microtiter plates

Injection volume:

1 - 300 µL

Injection volume accuracy:

>99.5%

Injection volume precision:

<0.3% RSD in full loop mode

<0.5% RSD in partial loop mode

<1% RSD in µL pickup mode

Injection overhead volume:

0 µL in µL pickup mode

Syringe volume:

250 µL standard

Temperature control range:

4 - 60 °C

COLUMN OVEN

Column capacity:

6 x analytical (1 x Tricorn 10/300 GL)

Temperature control range:

20 - 65 °C

OMNISEC REVEAL

Dimensions (W, D, H):

420, 640, 600 mm

Weight:

40 kg

Detector temperature range:

20 - 65 °C

Software:

OMNISEC software v10 (or later)

Data acquisition rate:

100 Hz

Patents:

US20140060162A1 & EP2619543Ba "Modular capillary bridge viscometer"

US20140144214A1 & EP2619544A1 "Automatically balanced capillary bridge viscometer"

Detector 1

Detector:

Light scattering

Measurement principle:

RALS 90° angle, LALS 7° angle

Light source:

50 mW, 640 nm laser

Cell volume:

18 µL

Dynamic range:

2500 mV

Baseline noise:

<0.1 mV

Baseline drift:

<0.2 mV/hr

Detector 2

Detector:

Differential refractive index

Measurement principle:

Deflection

Cell volume:

12 µL

Dynamic range:

±2.5 x 10^-4 RIU

Baseline noise:

<10^-7 RIU

Baseline drift:

<3 x 10^-7 RIU/hr

Detector 3

Detector:

Viscometer

Measurement principle:

4-capillary Wheatstone bridge with self-balancing mechanism and user-exchangeable capillaries

Transducers:

Firmware-based transducer overpressure protection

Detector volume:

17 µL/capillary

Differential pressure baseline noise:

0.3 Pa

Inlet pressure dynamic range:

100 kPa

Inlet pressure baseline noise:

0.01 kPa

Baseline drift:

<0.2 kPa/hr

Detector 4

Detector:

Diode-array-based UV/Vis spectrometer

Wavelength:

190 - 900 nm

Number of wavelengths:

1024

Wavelength accuracy:

<1 nm

Wavelength resolution:

0.6 nm

Cell volume:

7.5 µL

Path length:

10 mm

Baseline noise:

2 x 10^-5 AU

Baseline drift:

5 x 10^-4 AU/hr

Measurement type 1

Measurement type:

Absolute molecular weight

Measurement range:

200 - >10⁷ g/mol

Minimum quantifiable mass:

100 ng of 100kDa molecular weight polystyrene in THF

Measurement principle:

Light scattering (LALS or RALS)

Measurement type 2

Measurement type:

Intrinsic viscosity

Minimum quantifiable mass:

1 µg of 100kDa molecular weight polystyrene in THF

Measurement principle:

4-capillary Wheatstone bridge

Measurement type 3

Measurement type:

Concentration

Minimum quantifiable mass:

100 ng of 100kDa molecular weight polystyrene in THF

Measurement principle:

Differential refractive index detection

Operating environment

Temperature:

15 - 30 °C

Software

Minimum computer specification:

Windows 7 Professional 64 bit, 4th Gen Intel(R) Core I7 Processor (Quad Core HT, 3.4 GHz Turbo, w/ HD Graphics), 8 GB 1600 MHz DDR3 memory, 500 GB 3.5 inch SATA (7200 RPM) HDD, Full-HD monitor

Accessories

OMNISEC

Support

Protect your investment and ensure optimized performance at all times with Malvern Panalytical's service plans.

When you purchase a Malvern Panalytical product we understand that this is just the first stage of a working relationship that will last for the lifetime of the instrument. Depending on your needs, Malvern Panalytical will provide the support for your business.

Help Desk

• Telephone and email support.
• Operators who take responsibility for your call and ensure your query is answered.

Training

• User training events

Software downloads

• A simple mechanism for receiving updates and new features

Malvern Panalytical's service options- choose the right plan for you:

*including labour & travel costs***available at an additional cost

Key applications

The Malvern Panalytical OMNISEC can be used in many industry applications, including:

With OMNISEC you can…	Of…
Control strength, toughness, durability, brittleness, processability and branching	Polystyrene, nylon, PET, PVC, polyester, nitrocellulose, resins, epoxys, polyurethanes, PVA
Measure degradation and drug delivery rates	PLA, PLGA, PEG, polycaprolocatone
Grade natural polymers and polysaccharides	Alginates, xanthans, Gum Arabic, starch derivatives (e.g. maltodextrin), cellulose derivatives (e.g. HEC), carageenan, gelatin, guar gum, chitosan, hyaluronic acid
Control formulation viscosity	Alkyds, acrylics, polyurethanes, polyesters, resins, epoxys, PVA,
Control food mouth-feel and gelling	Pectins, alginates, xanthans, Gum Arabic, guar gum, starch derivatives (e.g. maltodextrin), carageenans
Assess and predict activity by measuring molecular weight	Proteins, antibodies, mAbs
Predict and understand immunogenicity and efficacy	Aggregates of proteins, biopharmaceuticals, biologics and mAbs
Control half-life, cell permeability and understand crystallization of protein conjugates	PEGylated proteins, membrane proteins, antibody-drug-conjugates