Date recorded: October 04 2012

Duration: 49 minutes 14 seconds

Conventional calibration for proteins in SEC is the most popular method, however the integration of other detectors can give higher efficiency and be more accurate. These detectors can be integrated into your existing protein separation system
Table of contents
1. Additional Detection Methods for Conventional Calibration  
01:09
2. Additional Detection Methods for Conventional Calibration
00:22
3. Contents
00:45
4. INTRODUCTION TO GPC & SEC
00:07
5. Gel Permeation and Size Exclusion Chromatography
01:11
6. Expansion of an existing LC system
01:14
7. Hardware Schematic Conventional GPC
00:46
8. Conventional Calibration
01:12
9. Conventional Calibration – Calibration Curve
01:20
10. Conventional Calibration – Protein Results
01:39
11. Conventional Calibration
01:05
12. Overlay of Conventional Calibration Curves
00:31
13. Conventional Calibration
01:43
14. LIGHT SCATTERING DETECTION
00:15
15. Light Scattering
00:32
16. Principles of Light Scattering
00:49
17. Principles of Light Scattering
00:18
18. Angular Dependence
01:37
19. Light Scattering Detection
02:00
20. Light Scattering Detectors
01:18
21. Light Scattering Detectors
01:12
22. Zetasizer μV optical setup
00:35
23. OmniFACE
00:38
24. Zetasizer for batch and SEC Measurements
01:21
25. BSA chromatogram
00:55
26. Conventional Calibration – Protein Results
01:21
27. VISCOSITY DETECTION
00:11
28. Viscosity Detector
00:30
29. Universal Calibration
01:29
30. Universal Calibration
00:27
31. Relation Between Structure and Intrinsic Viscosity
00:59
32. Protein Conformational Changes
01:57
33. Viscosity Detector
00:21
34. Conventional Calibration – The issue
00:17
35. Universal Calibration
00:38
36. Universal Calibration Line
00:10
37. Universal Calibration
00:20
38. Protein Mark-Houwink Plot
01:06
39. Viscosity Detection
00:29
40. Viscosity Detectors
01:19
41. CONNECTION TO AN LC SYSTEM
00:13
42. Connection of Additional Detectors (270 Detector)
02:41
43. Connection of Additional Detectors (Zetasizer µV)
01:33
44. Summary
01:14
45. QUESTIONS?
00:28
46. Contact Information
00:22
47. Additional Detection Methods for Conventional Calibration
06:35