Date recorded: April 10 2014

Duration: 53 minutes 34 seconds

The measurement of the "absolute" molecular weight (or molar mass) of polymers and proteins is often mentioned and referenced but what does it mean? This talk will present a summary of the solution techniques available for measurement of molecular weight and discuss how "absolute" they can be considered to be. The focus will be on light scattering techniques, both in batch mode and when incorporated with chromatography systems.

Table of contents
1. Polymer Solution Characterisation - Part 1. "Absolute" molecular weight - fact or fiction?
04:45
2. Absolute molecular weight – fact or fiction?
01:26
3. Polymer is a composite of the Greek words poly and meros, meaning “many parts.”
00:53
4. Molecular weight
01:06
5. Polymer molecular weight - Polystyrene
00:44
6. Molecular weight
00:45
7. Molecular weight
01:51
8. Classical Determination of MW
01:38
9. Osmometry
02:16
10. End group analysis
01:19
11. Viscosity
01:58
12. Other Methods for Molecular Weight Determination
01:25
13. Light Scattering
01:15
14. Static light scattering theory
00:56
15. Anisotropic scatterers and angular dependence
01:09
16. Accounting for anisotropic scattering
01:44
17. Right-angle light scattering (RALS)
00:38
18. Low-angle light scattering
00:05
19. RALS/LALS
00:35
20. Multi-angle light scattering
00:52
21. The 3 approaches in plain English
00:50
22. Batch vs GPC/SEC measurement
01:38
23. Batch measurements
01:38
24. GPC/SEC - The beginnings of the technique
02:40
25. GPC/SEC - Detection and Calibration strategies(1) Conventional Calibration
01:31
26. GPC/SEC - Detection and Calibration strategies
01:19
27. GPC/SEC - Detection and Calibration strategies(2) Universal Calibration
01:36
28. Detection and Calibration strategies(3) Light Scattering Calibration
01:00
29. Absolute molecular weight?
00:56
30. GPC/SEC and GPC/SEC with Light Scattering
02:08
31. Absolute molecular weight?
09:34
32. Contact Information
01:24