Date recorded: June 25 2014

Duration: 57 minutes 44 seconds

Hyaluronic acid (HA) is a natural polysaccharide, that plays an important role in tissues, such as skin, tendons, muscles, and cartilage. As well as medical applications, It finds wide-spread use in cosmetics, particularly skin creams. In this presentation, we demonstrate how a suite of combined analytical techniques can give insight into the structural difference that affect the efficacy of the final products. The information revealed includes molecular size and charge, absolute molecular weight distribution, branching structure and rheological properties.

Table of contents
1. The Science of Beauty: Characterising Hyaluronic Acid - the molecule behind anti-aging creams
00:21
2. The Science of BeautyCharacterising Hyaluronic Acid – the molecule behind anti-aging creams
01:26
3. Agenda
00:59
4. Hyaluronic Acids
00:55
5. HA Chemistry
01:29
6. Samples analysed by GPC
01:12
7. HA Chemistry
01:41
8. Multi-detection GPC
01:16
9. Separation: SEC
01:14
10. Malvern Light Scattering range
01:04
11. SEC Instrument Schematic
00:52
12. Light Scattering Theory
01:24
13. Light Scattering Detector
00:42
14. Traditional Solution Viscosity Measurements
00:41
15. Viscometry
00:51
16. Traditional Solution Viscosity Measurements
02:39
17. Quantitative Comparison of Modified HA Structures
01:24
18. Quantitative Comparison of Modified HA Structures
00:55
19. The Kuhn–Mark–Houwink–Sakurada equation
01:01
20. Mark Houwink plot - Linear vs. Crosslinked
01:54
21. Structural Comparison of Modified HA Structures
01:22
22. Conformation plot - Linear vs. Crosslinked
01:43
23. Agenda
00:35
24. Critical overlap concentration (c*)
01:53
25. Determining values for c*
02:32
26. Coil overlap parameter c[η]
03:09
27. Dilute/semi-dilute behaviour c ≤ c*
03:14
28. Concentrated behaviour c > c*
02:52
29. Measuring polymer solutions using Microrheology
03:37
30. Brownian Motion, Particle Size and Viscosity
00:00
31. Mean Square Displacement
01:53
32. MSD Time Dependence on viscoelasticity
00:00
33. Extraction of rheological data
01:31
34. Extraction of rheological data
00:00
35. Different HA samples, same concentration
01:41
36. Different HA samples, same concentration
01:27
37. Same HA sample, different concentrations
00:43
38. Summary
00:52
39. Questions?
03:39
40. Contact Information
01:01