Optimizing Fluorescence for Nanoparticle Tracking Analysis Measurements

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Nanoparticle tracking analysis (NTA) is a technique for measuring particle size, distribution, and count based on light scattering and Brownian motion. Fluorescence-NTA is a subset of NTA measurements where particle size, distribution and count of fluorescently labelled particles are measured. This technique enables the characterization and the identification of a subset of particles (speciation/phenotyping) unlocking their potential for diagnostic and therapeutic applications.


  • Matt Brown - Applications Manager (AMEC), Pharmaceutical & Food Sector, Malvern Panalytical
  • Ronald Soriano - Field Applications Scientist – Biophysical Characterization, Malvern Panalytical

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Who should attend? 

  • This presentation is intended for scientists working with fluorescently labelled particles, particularly extracellular vesicles (EVs), lipidic particles and viral vectors for diagnostics and drug delivery applications.
  • Research scientists in the field of flow cytometry and fluorescence optical microscopy techniques.

What will you learn? 

  • This webinar highlights fluorescence NTA measurement.  
  • We will discuss what fluorescence NTA is and how to optimize it for different types of nanoparticles such as EVs, lipidic particles and viral vectors.
  • We will provide specific fluorescence NTA applications and examples of labelling protocols for EVs and Lipidic particles.

Characterizing Exosomes And Nanoparticles Webinar Series

In this three-part webinar series, we will explore several areas in the characterization of exosomes and other biologically-sourced nanoparticles, including an overview of techniques commonly used to characterize biologically sourced nanoparticles, with a special focus on nanoparticle tracking analysis (NTA). In addition, we will discuss, in-depth, the best practices to obtain quality NTA data, and how to optimize fluorescence NTA measurements.

Want to know more? 

Register for free for any sessions in our webinar series - and keep an eye out for the recordings afterwards too!