This webinar describes how a team at Sanofi Pasteur employ technology to understand and predict the stability of their vaccine formulations.
Firstly, you’ll learn how differential scanning calorimetry (DSC) is used to determine the thermodynamic and kinetic stability of proteins. Beyond the Tm value determined by DSC, the heating rate dependence of protein denaturation events is explored. Free energy barriers separating the native state from the non-functional forms were determined to rank formulations in term of kinetic stability.
Next, you’ll discover how nanoparticle tracking analysis (NTA) is used to count and size viral particles. During a forced degradation study of vaccines, increase in particle size polydispersity by NTA and loss of antigenicity by ELISA was concomitantly observed. Thus, the team demonstrated the concordance between NTA and the conventional ELISA. Accelerated stability data were used to accurately extrapolate the stability of vaccines for years. The use of appropriated kinetic models was successfully applied for protein stability predictions, expiry date estimations, and to evaluate the impact of temperature excursions (cold chain breaks).
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Natalia Markova & Didier Clenet - Guest Speaker from Sanofi Pasteur
Who should watch?
- Scientists and project leaders in academia and industry working to characterize proteins and develop protein-based vaccines and therapeutics.
What will you learn?
- Basic principles of DSC and its application to vaccine development.
- How to apply DSC to the characterization of recombinant multi-domain proteins.
- The Benefits of DSC compared to other thermal shift techniques.
- How to apply NTA to successfully predict protein stability.