For the ZetasizerµV instrument, the maximum size specification (radius) is a peak mode of 0.5µm with a maximum size of 1.0µm. This technical note contains details of the experimental procedures and results obtained from measurements of a NIST traceable polystyrene latex standard as demonstration of these maximum size specifications.
The polystyrene latex standard sample was prepared for measurement by adding 100µl of the latex to 10ml of the 10mM NaCl solution. The measurements were taken at 25°C
The intensity size distributions obtained from repeat measurements of the 1.0µm polystyrene latex dispersion are shown in figure 1. The distribution shows the low polydispersity measured for this monodisperse sample. At the maximum size limit, the concentration of the latex has to be sufficient to avoid number fluctuations.
Figure 2 shows the correlation functions of the latex, showing some baseline noise, but no evidence of significant number fluctuations. This demonstrates the capability of the Zetasizer µV to measure particles with a size mode of 0.5µm radius. To characterize this size, the system has a capability of measuring the size of the distribution up to a 1.0µm radius.
The results reported in this technical note confirm that the maximum size capability (radius) for the Zetasizer µV is a peak mode of 0.5µm with a maximum size of 1.0µm.