Requirements to characterise manufactured nanomaterials (MNM) under relevant biological and environmental exposure conditions have been well established. This is key to understanding particle fate and behaviour and ultimately in determining the available MNM dose in toxicity assessments. Variations in media properties can significantly alter particle behaviour. This drives a need for technology capable of characterising particles in complex matrices. NTA presents a tool capable of meeting these requirements and is demonstrated with a series of experiments performend with a range of particle types in relevant biological and environmental media providing temporal data of suspension properties.