Master ITC & DSC! Discover Past Blog Posts from MicroCal! Access Now According to Your Goals and Level!

by Malvern Panalytical, Wednesday, 22nd April 2020

The new fiscal year has begun!
However, many of you may be spending time in an environment where work from home and research activities are restricted due to the impact of the novel coronavirus. As you spend more time in front of your PC than usual, why not take this opportunity to relearn the questions you have had about ITC and DSC?

For those who want to learn or relearn the basics of MicroCal ITC/DSC

・List of Back Issues of the Road to Calorimeter Master

What can you learn?
Beginner-friendly insights into common questions and troubleshooting methods for ITC and DSC from a user perspective. Everything is actually based on the author’s real-life experiences.

Who is it recommended for?
・Those planning to use ITC or DSC
・Those who have started using ITC or DSC but are not confident yet in data interpretation or troubleshooting
・Those who couldn’t attend the instruction manual meetings and are progressing with measurements in their own way
・Those who have iTC200 or VP-DSC but have no one around to teach them how to use it

*This blog provides instructions based on the operation method of iTC200 and VP-DSC, but even users of other models can learn the basic principles and precautions.

For those who want to improve their ITC skills

・Checkpoints of MicroCal ITC data
・Why is the binding molar ratio n not equal to 1?
・Are control data really acting as controls?
・Enthalpy changes involving protons
・Latest trends of ITC in molecular interaction analysis 2018
・Latest trends of ITC in molecular interaction analysis 2019

What can you learn?
Deepen your understanding by revisiting the subject after acquiring some understanding of ITC, learning know-how that can’t be covered completely in instruction manual meetings.

Who is it recommended for?
・All MicroCal ITC users

For those who want to improve their DSC skills

・Tips for DSC measurements of proteins and biopolymers
・Must-see FAQ for MicroCal DSC users regarding the DSC system
・How is Tm determined in DSC Origin analysis software?
・Questions and answers regarding MicroCal DSC

What can you learn?
Deepen your understanding by revisiting the subject after acquiring some understanding of DSC, learning know-how that can’t be covered completely in instruction manual meetings.

Who is it recommended for?
・All MicroCal DSC users

For those who want to master ITC maintenance

・FAQ on MicroCal ITC maintenance
・Maintenance methods for various MicroCal ITC systems
・What to do when solution doesn’t flow through the MicroCal iTC200 pipette
・Replacing plunger tips of MicroCal iTC200/Auto-iTC200

What can you learn?
In the FAQ on MicroCal ITC maintenance, you can learn about the top 3 SOS issues faced by users as received by MicroCal support representatives and their troubleshooting methods. If you print the maintenance methods for various MicroCal ITC systems and place it near the system, anyone can conduct their daily maintenance.

Who is it recommended for?
・All MicroCal ITC users

For those who want to master DSC maintenance

・FAQ on MicroCal DSC maintenance
・Maintenance methods for various MicroCal DSC systems

What can you learn?
In the FAQ on MicroCal DSC maintenance, you can learn about the top 3 SOS issues faced by users as received by MicroCal support representatives and their troubleshooting methods. If you print the maintenance methods for various MicroCal DSC systems and place it near the system, anyone can conduct their daily maintenance.

Who is it recommended for?
・All MicroCal DSC users

For those utilizing or wanting to utilize ITC & DSC in drug discovery

・A must-read now! ‘Progress in Structural Chemistry of Proteins Creating the Next Generation of Biopharmaceuticals‘
This is an outlook on the next generation of biopharmaceuticals by Professor Kohei Tsumoto from the Graduate School of Engineering and Institute of Medical Science, the University of Tokyo, featured in the December 2014 issue of Nikkei Science!

・For those who wish to download the Japanese material on DSC application cases related to biopharmaceutical development in one go, click here.

・The “MicroCal helpful content for drug discovery” introduced over the past 6 sessions
This section introduces application notes, videos, and tips that serve as hints for measurements related to drug discovery.

・Helpful Content Vol.1
① DSC Application Note
Investigation of stability in the antibody Fab region for enhancing biopharmaceutical development using DSC

② DSC Webinar (English)
Value of DSC in characterization and optimization of protein stability as compared to other thermal stability assays
(Role of DSC in characterization and optimization of protein stability compared to other stability assessment techniques)

③ DSC Tips
DSC Q&A

・Helpful Content Vol.2
① ITC White Paper (English)
Addressing the needs of drug discovery with the MicroCal PEAQ-ITC instruments (Approach to meet the needs of drug discovery using MicroCal PEAQ-ITC systems)

② ITC YouTube (English)
MicroCal PEAQ-ITC – the live launch (Introduction video of MicroCal PEAQ-ITC software)

③ – ITC Tips
ITC Data Checkpoints

・Helpful Content Vol.3
① DSC Application Note
Promoting the development of liquid formulations for protein-based biopharmaceuticals using Differential Scanning Calorimetry

② DSC YouTube (Japanese)
MicroCal PEAQ DSC, a DSC for the Regulated Environment (Introduction video of PEAQ-DSC customized for regulated environments)

③ DSC Tips
How is Tm determined using DSC Origin software?

・Helpful Content Vol.4
① ITC Application Note
Diverse applications of Malvern MicroCal Auto-iTC200 in FBDD

② ITC Webinar (English)
Biophysical characterization of epigenetic interactions and associated drug discovery using Isothermal Titration Calorimetry
(Biophysical evaluation of epigenetic interactions and related drug discovery using ITC)

③ ITC Tips
ITC Measurement Flowchart

・Helpful Content Vol.5
① DSC Application Note
DSC-related research on drug pre-formulation and stability of biopharmaceuticals

② DSC YouTube (English)
MicroCal DSC – Formulation stability testing (PEAQ DSC for formulation stability testing)

③ DSC Tips
Common troubleshooting methods in DSC

・Helpful Content Vol.6
① ITC/DSC Application Note
Targeting protein/protein interactions in drug design (Targeting protein/protein interactions in drug design)

② ITC YouTube (English)
Elucidating Epigenetic Interactions using ITC (Clarifying epigenetic interactions using ITC)

③ ITC Tips
Commonly encountered issues and their solutions in ITC

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This article may have been translated automatically