Date recorded: November 16 2011

Duration: 01 hours 01 minutes 33 seconds

This overview of GPC/SEC gives examples of polyolefins, proteins and biopolymers that benefit from this powerful analytical tool. Applications range from standard macromolecular characterization to advanced triple detection
Table of contents
1. From the applications laboratory What can GPC do for me?
02:03
2. From The Application Lab – What Can GPC Do For Me?
00:20
3. Abstract
00:41
4. Outline
00:57
5. Defining Some Terms
01:00
6. Defining Some Terms
01:38
7. What is Chromatography?
00:47
8. Size Exclusion Chromatography
01:44
9. The Separation Process
01:36
10. Why Use Size Exclusion Chromatography?
01:51
11. Molecular Weight Moments
00:48
12. Molecular Weight Moments – Distribution Averages
00:54
13. Understanding Your Sample
02:03
14. Components of an SEC System
02:01
15. Components of an SEC System
00:09
16. SEC/GPC Data Processing Options
00:57
17. Conventional Calibration
00:10
18. Conventional Calibration – Detectors from Viscotek
01:03
19. Concentration Detectors
00:35
20. Creating a Calibration Curve
00:50
21. Conventional GPC Systems
00:25
22. Overlay of Conventional Calibration Curves
00:46
23. Conventional Calibration
00:55
24. Conventional Calibration - Summary
00:40
25. Universal Calibration
00:19
26. Four-Capillary viscometer
00:51
27. Universal Calibration
01:07
28. Universal Calibration – PMMA 64 kDa Narrow Standard
01:21
29. Universal Calibration Conclusions
01:09
30. Triple Detection
00:15
31. Static Light Scattering – Rayleigh Theory
00:28
32. Static Light Scattering Summary
00:51
33. Triple Detection
02:16
34. Process Prediction Based on Structure
01:24
35. Long-Chain Branching
00:22
36. How is LCB Determined?
00:55
37. Structural Differences in Cellulose Excipients
01:02
38. Density Changes in CPVC -QA/QC
00:53
39. Applications – Variables To Consider
01:58
40. Aminated Chitosan – Cationic Column Selection
00:41
41. Polyalkylacrylamide - Inert Column Selection
00:48
42. PET in HFIPA – Inert Column Selection
00:55
43. HPMC in Acetic Acid – Glycosylated Column Selection
00:58
44. Polycarbonate
01:48
45. Functionalizing Chitosan
00:47
46. Functionalizing Chitosan
00:31
47. HT-GPC Applications
00:05
48. Polymers For High Temperature GPC/SEC
00:35
49. Polyethylene (PE) and Polypropylene (PP)
01:07
50. PE and PP at 140 °C in TCB
01:17
51. PP at 160 °C in TCB
01:53
52. Protein Analysis
00:07
53. Intrinsic Viscosity for Proteins
00:36
54. Tetra Detection GPC for Proteins - BSA
00:35
55. BSA – Molecular Weight Determination of Complexes
00:44
56. Untitled
00:35
57. BSA Aggregation – Chromatography Temp. = 45 °C
00:37
58. Compositional Analysis
00:51
59. PEGylated Proteins – Compositional Analysis
00:34
60. Untitled
00:33
61. Conclusions
01:23
62. Contact Information
03:29