Date recorded: December 06 2016
Duration: 31 minutes 42 secondsGel Permeation Chromatography (GPC), also known as Size Exclusion Chromatography (SEC), is a very popular analytical tool for characterizing natural and synthetic polymers. Advanced GPC/SEC analysis using multi-detectors is increasingly being used to measure absolute molecular weight independent of column retention volume and to add other measurements such as those of intrinsic viscosity and hydrodynamic radius. With this information, scientists can accurately study how changes in molecular weight and structure affect polymer performance.
Advances in detector technology have enabled more accurate and information-rich measurements, but the quality of those measurements is still dependent on the quality of the separation, which itself remains dependent on the column. Good resolution depends on matching the column pore size to the sample of interest and an ideal separation requires no interaction between the sample and the column material. Additionally, in order to maximize light scattering signal-to-noise, columns should be stable over time and not shed any particulates, to which the light scattering detector will be very sensitive.
From the detector standpoint, inter-detector distances should be reduced to minimize peak dispersion. This webinar discusses how high quality columns and detectors both contribute to high quality GPC/SEC measurements. It also discusses how the two interact with each other and show data to demonstrate some ideal measurements as well as showing some of the symptoms of poor measurements while discussing the possible causes.
Table of contents
3. Optimizing GPC/SEC measurements by combining high-performance columns and powerful advanced detectors
4. Introduction to GPC and multi-detection
5. HPLC Brief History
6. HPLC Brief History
7. Separation theory
8. Separation theory
9. Separation theory
10. Size Separation
11. Column considerations
12. Column considerations
13. Multi-detector GPC/SEC
14. The multi-detection pyramid
15. Multi-detector module – OMNISEC REVEAL
16. What interplay is there between separation & detection?
17. Band Broadening
18. Band broadening
19. Hardware improvements
20. Hardware improvements
21. Light scattering noise from column shedding
22. How can we address light scattering noise issues?
23. LB (ALS) columns
24. Summary of multi-detector GPC
25. Hyaluronic acid
26. Hyaluronic Acid
27. HA comparisons
28. HA comparisons
29. Hyaluronic Acid – Linear and Crosslinked
33. PLGA 50:50 - 8 repeat injections
37. Thank you for your attentionAny questions?Please type your questions in using the Q&A panel on the right side of your screen
38. Contact Info