Date recorded: December 06 2016

Duration: 31 minutes 42 seconds

Gel Permeation Chromatography (GPC), also known as Size Exclusion Chromatography (SEC), is a very popular analytical tool for characterizing natural and synthetic polymers. Advanced GPC/SEC analysis using multi-detectors is increasingly being used to measure absolute molecular weight independent of column retention volume and to add other measurements such as those of intrinsic viscosity and hydrodynamic radius. With this information, scientists can accurately study how changes in molecular weight and structure affect polymer performance.

Advances in detector technology have enabled more accurate and information-rich measurements, but the quality of those measurements is still dependent on the quality of the separation, which itself remains dependent on the column. Good resolution depends on matching the column pore size to the sample of interest and an ideal separation requires no interaction between the sample and the column material. Additionally, in order to maximize light scattering signal-to-noise, columns should be stable over time and not shed any particulates, to which the light scattering detector will be very sensitive.

From the detector standpoint, inter-detector distances should be reduced to minimize peak dispersion. This webinar discusses how high quality columns and detectors both contribute to high quality GPC/SEC measurements. It also discusses how the two interact with each other and show data to demonstrate some ideal measurements as well as showing some of the symptoms of poor measurements while discussing the possible causes.
Table of contents
1. Welcome
00:15
2. Untitled
02:02
3. Optimizing GPC/SEC measurements by combining high-performance columns and powerful advanced detectors
00:08
4. Introduction to GPC and multi-detection
00:07
5. HPLC Brief History
00:32
6. HPLC Brief History
00:44
7. Separation theory
00:52
8. Separation theory
00:29
9. Separation theory
00:23
10. Size Separation
01:11
11. Column considerations
00:40
12. Column considerations
00:41
13. Multi-detector GPC/SEC
01:51
14. The multi-detection pyramid
01:22
15. Multi-detector module – OMNISEC REVEAL
01:37
16. What interplay is there between separation & detection?
00:48
17. Band Broadening
01:47
18. Band broadening
00:55
19. Hardware improvements
00:27
20. Hardware improvements
00:53
21. Light scattering noise from column shedding
00:42
22. How can we address light scattering noise issues?
00:19
23. LB (ALS) columns
00:34
24. Summary of multi-detector GPC
00:44
25. Hyaluronic acid
00:12
26. Hyaluronic Acid
00:50
27. HA comparisons
01:00
28. HA comparisons
00:23
29. Hyaluronic Acid – Linear and Crosslinked
02:02
30. PLGA
00:07
31. PLGA
00:44
32. PLA
01:23
33. PLGA 50:50 - 8 repeat injections
00:36
34. PLA/PLGA
00:50
35. PLA/PLGA
00:29
36. Summary
01:13
37. Thank you for your attentionAny questions?Please type your questions in using the Q&A panel on the right side of your screen
00:15
38. Contact Info
01:35