NanoSight NS300
High resolution nanoparticle size and concentration data
The Malvern Panalytical NanoSight NS300 Instrument provides an easy-to-use, reproducible platform for nanoparticle characterization.
The NS300 allows rapid analysis of the size distribution and concentration of all types of nanoparticles from 0.01 - 1 µm* in diameter, depending on the instrument configuration and sample type.
With the ability to be supplied with interchangeable laser modules and the introduction of a motorized filter wheel means different fluorescent labels can be analyzed. Sample temperature is fully programmable through the Nanoparticle Tracking Analysis (NTA) software.
*sample and system configuration dependent
Features and benefits
This versatile instrument offers the major benefit of minimum user set-up, enhanced concentration measurements via advanced algorithms and fluorescence detection capability providing truly holistic analysis. The fully software-controlled filter wheel means automated analysis of fluorescent markers can be performed while walk-away NTA measurements for multiple samples can be performed when used with the Sample Assistant accessory
- Measure over 1000 particles in just 60 seconds.
- Particle by particle analysis for high resolution size and concentration.
- Visual validation of results gives extra confidence.
- User friendly software with easy set up of SOPs for routine use.
- Minimal sample preparation.
- On board temperature control.
- Choice of laser wavelengths.
- Add up to 5 additional filters in a motorized wheel for flexible fluorescence detection capability.
- Complies with Industry Standards ISO 19430:2016 and ASTM E 2834:2012
The NanoSight NS300 uses the technology of Nanoparticle Tracking Analysis (NTA). This technology utilizes the properties of both light scattering and Brownian motion in order to obtain the size distribution and concentration measurement of particles in liquid suspension. A laser beam is passed through the sample chamber, and the particles in suspension in the path of this beam scatter light in such a manner that they can easily be visualized via 20x magnification microscope onto which is mounted a camera. The camera operates at 30 frames per second (fps), capturing a video file of the particles moving under Brownian motion. The software tracks many particles individually and using the Stokes-Einstein equation calculates their hydrodynamic diameters. The measurement is controlled via Standard Operating Procedures which are set with a user-friendly software interface.
Key applications
The Malvern Panalytical NanoSight NS300 can be used in many key applications, including:
Extracellular Vesicles (EV)
Whilst the research into the generation and functions of extracellular vesicles is still evolving, it is important to monitor and control their isolation and purification. NanoSight NTA provides quick and easy characterization of both the size and concentration of vesicles in aqueous buffers, giving confidence in the quality of the sample used in any downstream experiments, whilst the use of fluorescence can assist in elucidating the origin subpopulations of EVs.
Whitepaper: NTA Exosomes and Microvesicles
Virus and Vaccines
Manufacture of vaccines requires controlled production processes to ensure that the materials can be appropriately dosed and recognised by the immune system. NTA assists in the optimization of manufacturing processes by ensuring the size distribution of the vaccine is maintained. As NTA is also able to count and size viruses, it can be used as a faster alternative to titre assays.
Application Note: Total virus titer in minutes
Drug Delivery & Gene Therapy
With drug delivery and gene therapy products, 70-150nm is the typical size of particles required to ensure effective disease targeting in the clinical. NTA is ideally suited to study the size of these particles from early stage research through to candidate screening, formulation development and clinical batch monitoring whilst the concentration measurement allows for dose determination of the final product as well as for use in in vitro and in vivo assays.
Whitepaper: NTA Applications in drug delivery
Biotherapeutics
Temperature, pH change, agitation, shear and time all impact the stability of biotherapeutic proteins, causing denaturation and aggregation which can cause loss of efficacy and the potential for an unwanted immune response. NTA provides high resolution size distributions of sub-visible aggregates in a formulation for safety and quality assurance.
Nanomaterials/colloids/toxicology
As nanomaterials are incorporated in to everyday items, they are coming to the attention of regulatory bodies. There is a requirement to fully characterize these particles, their properties and contribution in the final product. NanoSight can provide a number-based concentration for these nanomaterials and high-resolution size distributions for those working at the nanoscale in industrial, environmental and toxicological fields.
Ultrafine bubbles
In recent years work on ultrafine bubbles has been gathering momentum for use in industrial cleaning and water treatment, agriculture and food, medical applications amongst other. Their relatively low concentration and small size are a challenge for many traditional nanoparticle characterization techniques whereas NTA is particularly adept at the detection and analysis of these bubbles.
Webinar: Nanobubbles: What are they, what are their uses and how can I measure them?
Specification
General
- Wavelength:
- 405nm (violet), 488nm (blue), 532nm (green) or 642nm (red).
- Temperature control range:
- 5°C below ambient to 50°C.
- Stage:
- Fixed stage.
- Focus:
- Computer controlled motorized focus.
- Camera:
- sCMOS.
- Fluorescence:
- Motorized filter wheel with choice of up to 5 filters.
- Dimensions (W, D, H):
- 400mm x 250mm x 400mm.
- Particle size:
10nm - 1000nm*
*Sample and system configuration dependent
- Concentration range:
- 106 to 109 particles per mL.
- Power:
- 100V - 240V
Accessories
NanoSight NS300
NanoSight Sample Assistant
Sample storage and walk-away NTA measurements
The NanoSight Sample Assistant is a 96-well plate-based autosampler accessory with a bespoke software suite allowing for walk-away NTA measurements.NanoSight syringe pump
The Malvern Panalytical NanoSight syringe pump is available for all Malvern Panalytical NanoSight systems, enabling analysis in constant flow for improved sample statistics and measurement of photo-bleaching fluorescent samples.
Support
Protect your investment and ensure optimized performance at all times with Malvern Panalytical's service plans.
When you purchase a Malvern Panalytical product we understand that this is just the first stage of a working relationship that will last for the lifetime of the instrument. Depending on your needs, Malvern Panalytical will provide the support for your business.
Help Desk
- Telephone and email support.
- Operators who take responsibility for your call and ensure your query is answered.
Training
Software downloads
Malvern Panalytical's service options- choose the right plan for you:
Malvern Panalytical Platinum Plan
For laboratories where maximizing instrument up-time is critical to its daily performance. Our highest level of response and the 'all inclusive' price plan takes care of those unexpected repair bills should the unfortunate occur.
Malvern Panalytical Gold Plan
Enhance your laboratory productivity by maintaining instrument efficiency. Still receive that priority response, combined with specialist technical and software support, we'll keep your instrument performance on track to deliver.
Malvern Panalytical Bronze Visit
For laboratories looking to optimize instrument performance, regular preventative maintenance is essential.
| Platinum Plan | Gold Plan | Bronze Visit | |
|---|---|---|---|
| Annual PM/PV Test | yes | yes | no |
| Telephone / Email Support | yes | yes | no |
| Priority Response | yes | yes | no |
| Emergency Breakdown visits* | yes | yes | no |
| Parts Included | yes | no | no |
| Technical & Software Support | yes | yes | yes |
| IQ/OQ (Pharma)*** | yes | yes | yes |
*including labour & travel costs***available at an additional cost
